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Drug Res (Stuttg) 2021; 71(07): 372-378
DOI: 10.1055/a-1488-5904
Original Article

Mechanistic Evaluation of Linalool Effect against Renal Ischemia- Reperfusion Injury in Rats

Mohammad-Ghasem Golmohammadi 
1   Department of Anatomy, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
,
Shokofeh Banaei 
2   Department of Physiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
,
Ehsan Azimian 
3   Department of Linguistics and Foreign Languages, Payame Noor University, Tehran, Iran
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Abstract

Background Kidney ischemia reperfusion (IR) is an important cause of renal dysfunction. The hypoxic conditions in ischemic damage result in the formation of free radicals and apoptotic death of renal cells. We evaluated the renoprotective effects of linalool in IR- induced renal injury.

Methods Wistar rats were divided into three groups of six rats; namely, control group, IR group, and linalool + IR group. The animals were unilaterally nephrectomized and subjected to 45 min of renal pedicle occlusion followed by 24 h reperfusion. Linalool (40mg/kg) was administered before ischemia. After 24h reperfusion, the kidney tissues were obtained for detection of miR-21, HSP 70 and caspase-3 expression levels and histological studies. Also, the blood samples were collected for the measurement of biochemical parameters.

Results IR significantly increased the expression of miR-21, HSP70 and capase-3 and the serum levels of BUN-Cr, ALT, AST and ALP enzymes. Furthermore, histological findings of the IR group confirmed that there were acute tubular necrosis and lymphocyte infiltration in the renal tissues. Treatment with linalool improved the renal function and morphological changes.

Conclusion It seems that linalool could exert a nephroprotective effect via a number of mechanisms in renal IR injury.

Key words

Linalool - Ischemia-reperfusion - Kidney


Publication History

Received: 25 November 2020

Accepted: 19 April 2021

Article published online:
21 May 2021

© 2021. Thieme. All rights reserved.

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  • References

  • 1 Amani H., Habibey R., Hajmiresmail S.J. et al. Antioxidant nanomaterials in advanced diagnoses and treatments of ischemia reperfusion injuries. Journal of Materials Chemistry B 2017; 5 (48) p 9452-p 9476
    CrossrefPubMedGoogle Scholar
  • 2 Cura-Esquivel I, Delgado-Chávez E.N., García-Narro J. H.H. et al. Attenuation of pro-inflammatory cytokines and oxidative stress by misoprostol in renal ischemia/reperfusion in rats. Pharmazie 2018; 73 (09) p 537-p 540
    PubMedGoogle Scholar
  • 3 Banaei S. Novel role of microRNAs in renal ischemia reperfusion injury. Ren Fail 2015; 37 (07) p 1073-p 1079
    CrossrefPubMedGoogle Scholar
  • 4 Epure A., Pârvu Alina E.E., Vlase L. et al. Phytochemical Profile, Antioxidant, Cardioprotective and Nephroprotective Activity of Romanian Chicory Extract. Plants 2021; 10 (01) p 64
    CrossrefPubMedGoogle Scholar
  • 5 Mazani M., Rezagholizadeh L., Shamsi S. et al. Protection of CCl4-induced hepatic and renal damage by linalool. Drug and Chemical Toxicology 2020; p 1-p 9
    CrossrefPubMedGoogle Scholar
  • 6 Zheng X.-H., Liu C.-P., Hao Z.-G. et al. Protective effect and mechanistic evaluation of linalool against acute myocardial ischemia and reperfusion injury in rats. RSC advances 2017; 7 (55) p 34473-p 34481
    CrossrefPubMedGoogle Scholar
  • 7 Iwasaki K., Zheng Y.-W., Murata S. et al. Anticancer effect of linalool via cancer-specific hydroxyl radical generation in human colon cancer. World journal of gastroenterology 2016; 22 (44) p 9765
    CrossrefPubMedGoogle Scholar
  • 8 Gunaseelan S., Balupillai A., Govindasamy K. et al. Linalool prevents oxidative stress activated protein kinases in single UVB-exposed human skin cells. PLoS One 2017; 12 (05) p e0176699
    CrossrefPubMedGoogle Scholar
  • 9 Kalantari H., Jalali M., Jalali A. et al. Protective effect of Cassia fistula fruit extract against bromobenzene-induced liver injury in mice. Hum Exp Toxicol 2011; 30 (08) p 1039-p 1044
    CrossrefPubMedGoogle Scholar
  • 10 Huang S., Lin Y., Liang Z. et al. Erythropoietin helix B surface peptide modulates miR-21/Atg12 axis to alleviates cardiomyocyte hypoxia-reoxygenation injury. Am J Transl Res 2019; 11 (04) p 2422-p 2430
    PubMedGoogle Scholar
  • 11 Wang F., Shang Y, Zhang R. et al. A SIRT1 agonist reduces cognitive decline in type 2 diabetic rats through antioxidative and anti‑inflammatory mechanisms. Mol Med Rep 2019; 19 (02) p 1040-p 1048
    PubMedGoogle Scholar
  • 12 Liu W., Chen X., Zhang Y. Effects of microRNA-21 and microRNA-24 inhibitors on neuronal apoptosis in ischemic stroke. Am J Transl Res 2016; 8 (07) p 3179-p 3187
    PubMedGoogle Scholar
  • 13 Banaei S., Ahmadiasl N., Alihemmati A. Comparison of the Protective Effects of Erythropoietin and Melatonin on Renal Ischemia-Reperfusion Injury. Trauma Mon 2016; 21 (03) p e23005
    CrossrefPubMedGoogle Scholar
  • 14 Banaei S. The effect of 5-aminosalicylic acid on renal ischemia-reperfusion injury in rats. Indian J Pharmacol 2016; 48 (02) p 196-p 199
    CrossrefPubMedGoogle Scholar
  • 15 Golmohammadi M.G., Banaei S. Vitamin D3 and erythropoietin protect against renal ischemia-reperfusion injury via heat shock protein 70 and microRNA-21 expression. Sci Rep 2020; 10 (01) p 20906
    CrossrefPubMedGoogle Scholar
  • 16 Golmohammadi M.-G., Ajam R., Shahbazi A. et al. Protective effect of vitamin D3 and erythropoietin on renal ischemia/reperfusion-induced liver and kidney damage in rats. Journal of Herbmed Pharmacology 2020; 9: 3
    PubMedGoogle Scholar
  • 17 Ahmadiasl N., Banaei S., Alihemmati A. et al. The anti-inflammatory effect of erythropoietin and melatonin on renal ischemia reperfusion injury in male rats. Adv Pharm Bull 2014; 4 (01) p 49-p 54
    PubMedGoogle Scholar
  • 18 Xu H.X., Pan W., Qianv J.-F. et al. MicroRNA‑21 contributes to the puerarin‑induced cardioprotection via suppression of apoptosis and oxidative stress in a cell model of ischemia/reperfusion injury. Mol Med Rep 2019; 20 (01) p 719-p 727
    PubMedGoogle Scholar
  • 19 Xu X., Kriegel A. J., Liu Y. et al. Delayed ischemic preconditioning contributes to renal protection by upregulation of miR-21. Kidney Int 2012; 82 (11) p 1167-p 1175
    CrossrefPubMedGoogle Scholar
  • 20 Jiao X., Xu X., Fang Y. et al. miR-21 contributes to renal protection by targeting prolyl hydroxylase domain protein 2 in delayed ischaemic preconditioning. Nephrology (Carlton) 2017; 22 (05) p 366-p 373
    CrossrefPubMedGoogle Scholar
  • 21 Salloum F.N., Yin C., Kukreja R.C. Role of microRNAs in cardiac preconditioning. J Cardiovasc Pharmacol 2010; 56 (06) p 581-p 588
    CrossrefPubMedGoogle Scholar
  • 22 Amanvermez R., Acar E., Günay M. et al. Hsp 70, hsCRP and oxidative stress in patients with acute coronary syndromes. Bosn J Basic Med Sci 2012; 12 (02) p 102-p 107
    CrossrefPubMedGoogle Scholar
  • 23 Zhang L., Gao J., Chen T. et al. Microvesicles Derived from Human Embryonic Neural Stem Cells Inhibit the Apoptosis of HL-1 Cardiomyocytes by Promoting Autophagy and Regulating AKT and mTOR via Transporting HSP-70. 2019; 2019: p 6452684
    PubMedGoogle Scholar
  • 24 D'Amelio M., Cavallucci V., Cecconi F. Neuronal caspase-3 signaling: not only cell death. Cell Death Differ 2010; 17 (07) p 1104-p 1114
    CrossrefPubMedGoogle Scholar
  • 25 Pan Y., Guo M., Nie Z. et al. Colorimetric detection of apoptosis based on caspase-3 activity assay using unmodified gold nanoparticles. Chem Commun (Camb) 2012; 48 (07) p 997-p 999
    CrossrefPubMedGoogle Scholar
  • 26 Banaei S., Ahmadiasl N., Alihemmati A. Combination Anti-Apoptotic Effect of Erythropoietin and Melatonin on Ischemia Reperfusion-Induced Renal Injury in Rats. Acta Med Iran 2016; 54 (10) p 624-p 630
    PubMedGoogle Scholar