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Drug Res (Stuttg) 2020; 70(08): 360-366
DOI: 10.1055/a-1201-2436
Original Article

Protective Role of EGCG Against Malathion Induced Genotoxicity Using Human Lymphocytes

Seyed Mousa Taghavi
1   Toxicology and Poisoning Research Centre, Tehran University of Medical Sciences, Tehran, Iran
2   Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
,
Amir Shadboorestan
2   Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
,
Samin Sabzevari
1   Toxicology and Poisoning Research Centre, Tehran University of Medical Sciences, Tehran, Iran
2   Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
,
Maryam Gholami
1   Toxicology and Poisoning Research Centre, Tehran University of Medical Sciences, Tehran, Iran
2   Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
,
Razieh Keshavarz-maleki
1   Toxicology and Poisoning Research Centre, Tehran University of Medical Sciences, Tehran, Iran
2   Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
,
Hossein Mozdarani
3   Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
,
Seyed Nasser Ostad
1   Toxicology and Poisoning Research Centre, Tehran University of Medical Sciences, Tehran, Iran
2   Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
,
Omid Sabzevari
1   Toxicology and Poisoning Research Centre, Tehran University of Medical Sciences, Tehran, Iran
2   Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
› Author Affiliations Funding: This study was supported by a grant (No. 29479) from Vice Chancellor for Research, Tehran University of Medical Sciences, Tehran, Iran.
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Abstract

Background: Green tea (Camellia sinensis), which is the most common drink across the world after water, has many antioxidant properties. Epigallocatecin-3-gallate (EGCG) is a flavonoid which accounts for 33–50% of green tea solids. It functions as a powerful antioxidant, preventing oxidative damage in healthy cells, with antiangiogenic and antitumor activities and as a modulator of tumor cell response to chemotherapy. Malathion is an organophosphate pesticide which is widely used in agriculture, veterinary and industries. Oxidative stress has been identified as one of malathion’s main molecular mechanisms. The purpose of this study was to evaluate protective role of EGCG against malathion induced genotoxicity using human lymphocyte model. Blood samples from 8 non-smoker healthy volunteers with no history of chemotherapy were collected and divided into six groups: Control, EGCG (50 µM), EGCG (20 µM), Malathion (24 µM), EGCG (50 µM)+Malathion (24 µM) and EGCG (20 µM)+Malathion (24 µM). For genotoxicity assay, we employed micronuclei test. For antioxidant capacity evaluation, GSH content and MDA levels were measured. Malathion showed significant genotoxic damage compared to the intact lymphocytes, however, treatment with EGCG at both concentrations were reduced the genotoxic effect of malathion. Malathion induced lipid peroxidation, while pre-treatment with EGCG at both concentrations, significantly protected the lymphocytes against malathion induced lipid peroxidation. Malathion significantly reduced GSH content, but pre-treatment with EGCG significantly recovered GSH content. Overall this study demonstrated that EGCG (at both concentrations) significantly prevents human lymphocytes against malathion induced genotoxicity and oxidative damage.

Key words

anticancer drugs - toxicology - pharmacogenetics


Publication History

Received: 20 January 2020

Accepted: 08 June 2020

Article published online:
10 July 2020

© Georg Thieme Verlag KG
Stuttgart · New York

 

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