Abstract
Background Curcumin is a polyphenolic compound with numerous therapeutic
activities. There is no validated method available for the quantitative
estimation of curcumin in simulated nasal fluid.
Objective The aim of present investigation was to develop a simple and
precise UV visible spectrophotometric method for estimation of pure form of
curcumin in simulated nasal fluid.
Method Suitable solvent system was selected by estimation of curcumin at
UV maxima of 421nm in simulated nasal fluid with two surfactants (tween 80 and
sodium lauryl sulphate). The double beam UV visible spectrophotometer was used
for measurement of absorption. The selected solvent system was further validated
according to guidelines of international conference on harmonization (ICH), the
analytical parameter like linearity, precision and accuracy etc. were
studied.
Results Simulated nasal fluid with tween 80 at 1% concentration
satisfied all the conditions relative to Peak quality at the stated wavelength.
In developed method, curcumin was found to be linear over selected concentration
range of 5 to 60µg/ml with a correlation coefficient of 0.998.
The accuracy was found to be in range of
99.51 –100.223%.The precision was found to be less than
2 in terms of % RSD. The LOD & LOQ were 0.3657 & 1.109
respectively.
Conclusion The proposed method was found to be simple, sensitive and
precise. The most important this method can be used for routine quality control
analysis of curcumin with accuracy.
Key words
epidemiology - incidence - prevalence - prospective cohort
