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Drug Res (Stuttg) 2020; 70(08): 356-359
DOI: 10.1055/a-1193-4655
Original Article

Method Development and Validation of UV Spectrophotometric Method for the Quantitative Estimation of Curcumin in Simulated Nasal Fluid

Sherry Sharma
1   MM College of Pharmacy, MM (Deemed to be) University, Mullana, Ambala, Haryana, India
,
Jai Bharti Sharma
1   MM College of Pharmacy, MM (Deemed to be) University, Mullana, Ambala, Haryana, India
,
Shailendra Bhatt
1   MM College of Pharmacy, MM (Deemed to be) University, Mullana, Ambala, Haryana, India
,
Manish Kumar
1   MM College of Pharmacy, MM (Deemed to be) University, Mullana, Ambala, Haryana, India
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Abstract

Background Curcumin is a polyphenolic compound with numerous therapeutic activities. There is no validated method available for the quantitative estimation of curcumin in simulated nasal fluid.

Objective The aim of present investigation was to develop a simple and precise UV visible spectrophotometric method for estimation of pure form of curcumin in simulated nasal fluid.

Method Suitable solvent system was selected by estimation of curcumin at UV maxima of 421nm in simulated nasal fluid with two surfactants (tween 80 and sodium lauryl sulphate). The double beam UV visible spectrophotometer was used for measurement of absorption. The selected solvent system was further validated according to guidelines of international conference on harmonization (ICH), the analytical parameter like linearity, precision and accuracy etc. were studied.

Results Simulated nasal fluid with tween 80 at 1% concentration satisfied all the conditions relative to Peak quality at the stated wavelength. In developed method, curcumin was found to be linear over selected concentration range of 5 to 60µg/ml with a correlation coefficient of 0.998. The accuracy was found to be in range of 99.51 –100.223%.The precision was found to be less than 2 in terms of % RSD. The LOD & LOQ were 0.3657 & 1.109 respectively.

Conclusion The proposed method was found to be simple, sensitive and precise. The most important this method can be used for routine quality control analysis of curcumin with accuracy.

Key words

epidemiology - incidence - prevalence - prospective cohort


Publication History

Received: 09 April 2020

Accepted: 02 June 2020

Article published online:
23 June 2020

© Georg Thieme Verlag KG
Stuttgart · New York

 

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