Role of Basal Hormones on Sweat Rate and Sweat Na⁺  Loss in Elite Women Soccer Players

Abstract

We aimed to determine whether basal concentrations of testosterone, cortisol or the ratio testosterone/cortisol were related to sweat Na⁺ loss, sweat Na⁺ concentration ([Na⁺]) and sweat rate during exercise. Twenty-two female elite soccer players participated in the study. Testosterone and cortisol were measured in blood samples before exercise. Sweat samples were collected during a training session (~20°C, ~30% RH, and ~0.55 m/s of wind speed) to measure sweat [Na⁺]. Sweat rate was determined by considering the difference between post-and pre-body weight, along with the amount of liquid consumed. During exercise, sweat Na⁺ loss (0.33[0.19] g/h) and sweat rate (0.49[0.20] L/h) were related to basal testosterone concentration (1.4[0.4] pg/mL) (r=0.54; r=0.55, respectively; p<0.05), but not with basal cortisol concentration (119.2[24.2] ng/mL) nor testosterone/cortisol ratio (0.012[0.003]) (p>0.05). However, when Na⁺ loss was adjusted to sweat rate, no association was found between Na⁺ loss and testosterone (p>0.05). In addition, no differences were found between players with high vs. low Na⁺ loss adjusted to sweat loss in menstrual phase or intensity during exercise (p>0.05). In conclusion, these results suggest that in these specific environmental conditions, basal levels of testosterone might increase sweat rate and therefore, the amount of Na⁺ lost during exercise in elite women soccer players.

Key words

salty sweaters - hormone concentration - hydration status - menstrual phase

Publication History

Received: 05 August 2019

Accepted: 15 April 2020

Article published online:
26 May 2020

• References

• 1 Baker LB. Sweating rate and sweat sodium concentration in athletes: A review of methodology and intra/interindividual variability. Sports Med 2017; 47: 111-128
• 2 Lara B, Gallo-Salazar C, Puente C. et al. Interindividual variability in sweat electrolyte concentration in marathoners. J Int Soc Sports Nutr 2016; 13: 31
• 3 Buono MJ, Ball KD, Kolkhorst FW. Sodium ion concentration vs. sweat rate relationship in humans. J Appl Physiol (1985) 2007; (103) 990-994
• 4 Yoshida T, Shin-ya H, Nakai S. et al. Genomic and non-genomic effects of aldosterone on the individual variation of the sweat Na⁺  concentration during exercise in trained athletes. Eur J Appl Physiol 2006; 98: 466-471
• 5 Robertson MT, Boyajian MJ, Patterson K. et al. Modulation of the chloride concentration of human sweat by prolactin. Endocrinology 1986; 119: 2439-2444
• 6 Hew-Butler T, Hummel J, Rider BC. et al. Characterization of the effects of the vasopressin V2 receptor on sweating, fluid balance, and performance during exercise. Am J Physiol Regul Integr Comp Physiol 2014; 307: R366-R375
• 7 Del Coso J, Lara B, Salinero JJ. et al. CFTR genotype-related body water and electrolyte balance during a marathon. Scand J Med Sci Sports 2016; 26: 1036-1044
• 8 Brown MB, Haack KK, Pollack BP. et al. Low abundance of sweat duct Cl- channel CFTR in both healthy and cystic fibrosis athletes with exceptionally salty sweat during exercise. Am J Physiol Regul Integr Comp Physiol 2011; 300: R605-R615
• 9 Shirreffs SM, Sawka MN, Stone M. Water and electrolyte needs for football training and match-play. J Sports Sci 2006; 24: 699-707
• 10 Castro-Sepulveda M, Cancino J, Fernández-Verdejo R. et al. Basal serum cortisol and testosterone/cortisol ratio are related to rate of Na⁺  lost during exercise in elite soccer players. Int J Sport Nutr Exerc Metab 2019; 29: 659-663
• 11 Laube M, Bossmann M, Thome UH. Glucocorticoids distinctively modulate the CFTR channel with possible implications in lung development and transition into extrauterine life. PLoS One 2015; 10: e0124833
• 12 Khadijah Ramli NS, Giribabu N, Salleh N. Testosterone enhances expression and functional activity of epithelial sodium channel (ENaC), cystic fibrosis transmembrane regulator (CFTR) and sodium hydrogen exchanger (NHE) in vas deferens of sex-steroid deficient male rats. Steroids 2018; 138: 117-133
• 13 Kilding AE, Tunstall H, Wraith E. et al. Sweat rate and sweat electrolyte composition in international female soccer players during game specific training. Int J Sports Med 2009; 30: 443-447
• 14 Gibson JC, Stuart-Hill LA, Pethick W. et al. Hydration status and fluid and sodium balance in elite Canadian junior women’s soccer players in a cool environment. Appl Physiol Nutr Metab 2012; 37: 931-937
• 15 Peñailillo LE, Escanilla FA, Jury ER. et al. Differences in salivary hormones and perception of exertion in elite women and men volleyball players during tournament. J Sports Med Phys Fitness 2018; 58: 1688-1694
• 16 Ichinose-Kuwahara T, Inoue Y, Iseki Y. et al. Sex differences in the effects of physical training on sweat gland responses during a graded exercise. Exp Physiol 2010; 95: 1026-1032
• 17 Toering TJ, Gant CM, Visser FW. et al. Sex differences in renin-angiotensin-aldosterone system affect extracellular volume in healthy subjects. Am J Physiol Renal Physiol 2018; 314: F873-F878
• 18 Gagnon D, Crandall CG, Kenny GP. Sex differences in postsynaptic sweating and cutaneous vasodilation. J Appl Physiol (1985) 2013; (114) 394-401
• 19 Mukaibo T, Gao X, Yang NY. et al. Sexual dimorphisms in the transcriptomes of murine salivary glands. FEBS Open Bio 2019; 9: 947-958
• 20 Del Coso J, Estevez E, Baquero RA. et al. Anaerobic performance when rehydrating with water or commercially available sports drinks during prolonged exercise in the heat. Appl Physiol Nutr Metab 2008; 33: 290-298
• 21 Strazzullo P, Leclercq C. Sodium. Adv Nutr 2014; 5: 188-190
• 22 Moquin A, Mazzeo RS. Effect of mild dehydration on the lactate threshold in women. Med Sci Sports Exerc 2000; 32: 396-402
• 23 Bangsbo J, Iaia FM, Krustrup P. Metabolic response and fatigue in soccer. Int J Sports Physiol Perform 2007; 2: 111-127
• 24 Armstrong LE, Ganio MS, Casa DJ. et al. Mild dehydration affects mood in healthy young women. J Nutr 2012; 142: 382-388
• 25 Harriss DJ, Macsween A, Atkinson G. Ethical standards in sport and exercise science research: 2020 update. Int J Sports Med 2019; 40: 813-817
• 26 Janse de Jonge XA. Effects of the menstrual cycle on exercise performance. Sports Med 2003; 33: 833-851
• 27 Castro-Sepulveda M, Astudillo J, Letelier P. et al. Prevalence of dehydration before training sessions, friendly and official matches in elite female soccer players. J Hum Kinet 2016; 50: 79-84
• 28 Baker LB, Stofan JR, Hamilton AA. et al. Comparison of regional patch collection vs. whole body washdown for measuring sweat sodium and potassium loss during exercise. J Appl Physiol (1985) 2009; (107) 887-895
• 29 Baker LB, Ungaro CT, Barnes KA. et al. Validity and reliability of a field technique for sweat Na⁺  and K⁺  analysis during exercise in a hot-humid environment. Physiol Rep 2014; 2: e12007
• 30 Vescovi JD, Favero TG. Motion characteristics of women’s college soccer matches: Female Athletes in Motion (FAiM) study. Int J Sports Physiol Perform 2014; 9: 405-414
• 31 Buono MJ, Kolding M, Leslie E. et al. Heat acclimation causes a linear decrease in sweat sodium ion concentration. J Therm Biol 2018; 71: 237-240
• 32 Olson BR, Forman MR, Lanza E. et al. Relation between sodium balance and menstrual cycle symptoms in normal women. Ann Intern Med 1996; 125: 564-567
• 33 Baker LB, De Chavez PJ, Ungaro CT. et al. Exercise intensity effects on total sweat electrolyte losses and regional vs. whole-body sweat [Na+], [Cl-], and [K+]. Eur J Appl Physiol 2019; 119: 361-375