Wild Strawberry, Blackberry, and Blueberry Leaf Extracts Alleviate Starch-Induced Hyperglycemia in Prediabetic and Diabetic Mice

István Takács; András Szekeres; Ákos Takács; Dávid Rakk; Miklós Mézes; Ágnes Polyák; Lóránt Lakatos; Gyöngyi Gyémánt; Dezső Csupor; Krisztina J. Kovács; Szilamér Ferenczi

doi:10.1055/a-1164-8152

Planta Medica, Table of Contents

Planta Med 2020; 86(11): 790-799
DOI: 10.1055/a-1164-8152

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Wild Strawberry, Blackberry, and Blueberry Leaf Extracts Alleviate Starch-Induced Hyperglycemia in Prediabetic and Diabetic Mice

István Takács

¹Department of Microbiology, University of Szeged, Szeged, Hungary

,

András Szekeres

¹Department of Microbiology, University of Szeged, Szeged, Hungary

,

Ákos Takács

²Department of Food Engineering, University of Szeged, Szeged, Hungary

,

Dávid Rakk

¹Department of Microbiology, University of Szeged, Szeged, Hungary

,

Miklós Mézes

³Department of Nutrition, Szent István University, Gödöllő, Hungary

,

Ágnes Polyák

⁴Laboratory of Molecular Neuroendocrinology, Institute of Experimental Medicine, Budapest, Hungary

,

Lóránt Lakatos

⁵Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary

⁶Present address: Biological Research Center, Szeged, Hungary

,

Gyöngyi Gyémánt

⁷Department of Inorganic and Analytical Chemistry, University of Debrecen, Debrecen, Hungary

,

Dezső Csupor

⁸Department of Pharmacognosy, Faculty of Pharmacy, University of Szeged, Szeged, Hungary

,

Krisztina J. Kovács^*

⁴Laboratory of Molecular Neuroendocrinology, Institute of Experimental Medicine, Budapest, Hungary

,

Szilamér Ferenczi^*

⁴Laboratory of Molecular Neuroendocrinology, Institute of Experimental Medicine, Budapest, Hungary

› Author Affiliations

Abstract

Intestinal α-glucosidase and α-amylase break down nutritional poly- and oligosaccharides to monosaccharides and their activity significantly contributes to postprandial hyperglycemia. Competitive inhibitors of these enzymes, such as acarbose, are effective antidiabetic drugs, but have unpleasant side effects. In our ethnopharmacology inspired investigations, we found that wild strawberry (Fragaria vesca), blackberry (Rubus fruticosus), and European blueberry (Vaccinium myrtillus) leaf extracts inhibit α-glucosidase and α-amylase enzyme activity in vitro and are effective in preventing postprandial hyperglycemia in vivo. Toxicology tests on H9c2 rat embryonic cardiac muscle cells demonstrated that berry leaf extracts have no cytotoxic effects. Oral administration of these leaf extracts alone or as a mixture to normal (control), obese, prediabetic, and streptozotocin-induced diabetic mice attenuated the starch-induced rise of blood glucose levels. The efficiency was similar to that of acarbose on blood glucose. These results highlight berry leaf extracts as candidates for testing in clinical trials in order to assess the clinical significance of their effects on glycemic control.

Key words

diabetes - α-glucosidase - α-amylase - Fragaria vesca - Rubus fruticosus - Vaccinium myrtillus - Rosaceae - Ericaceae

Full Text

References

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