Planta Med 2019; 85(04): 274-281
DOI: 10.1055/a-0762-0885
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

PPARα and γ Activation Effects of New Nor-triterpenoidal Saponins from the Aerial Parts of Anabasis articulata

Riham Salah El Dine
1   Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt
,
Hossam M. Abdallah
1   Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt
2   Department of Natural Products, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia
,
Zeinab A. Kandil
1   Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt
,
Ahmed A. Zaki
3   National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, Mississippi, USA
4   Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt
,
Shabana I. Khan
3   National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, Mississippi, USA
,
Ikhlas A. Khan
3   National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, Mississippi, USA
› Author Affiliations
Further Information

Publication History

received 01 June 2018
revised 05 October 2018

accepted 14 October 2018

Publication Date:
25 October 2018 (online)

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Abstract

Anabasis articulata, traditionally used to treat diabetes, is rich in saponin content. This study was performed to investigate the agonistic effect of its saponins on peroxisome proliferator-activated receptor-α and peroxisome proliferator-activated receptor-γ in human hepatoma (HepG2) cells to explore the possibility of the involvement of these nuclear receptors in the mechanism of the antidiabetic effect of the plant. Chemical investigation of the n-butanol fraction resulted in the isolation of three new and one known 30-noroleanane triterpenoid saponins. The structures of the new compounds were elucidated as 3β-hydroxy,23-aldehyde-30-norolean-12,20(29)-dien-28-oic acid-28-O-β-D-glucopyranosyl ester (1), 3β-O-D-galactopyranosyl-23-aldehyde-30-norolean-12,20(29)-dien-28-oic acid-28-O-β-D-glucopyranosyl ester (2), and 3β-O-D-xylopyranosyl-30-norolean-12,20(29)-dien-28-oic acid 28-O-β-D-glucopyranosyl ester (3), while the known 30-nortriterpenoidal saponin was identified as boussingoside E (4). Although, the isolated saponins (1 – 4) did not show > 1.5-fold activation of peroxisome proliferator-activated receptor-γ, but two of them (1 and 3) activated peroxisome proliferator-activated receptor-α to the higher extents of 2.25- and 1.86-fold, respectively. These results suggest that the reported antidiabetic action of the isolated saponins may not solely involve the activation of peroxisome proliferator-activated receptor-γ. However, the agonistic activity of the n-butanol fraction of A. articulata (1.71-fold induction) and two of its saponins (1 and 3) towards peroxisome proliferator-activated receptor-α may be beneficial in the cardiovascular condition that is closely associated with diabetes and other metabolic disorders.

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