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Drug Res (Stuttg) 2018; 68(12): 696-703
DOI: 10.1055/a-0626-7003
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Protective but Non-Synergistic Effects of Nigella Sativa and Vitamin E against Cisplatin-Induced Renal Toxicity and Oxidative Stress in Wistar Rats

Abdulwasiu A. Busari
1   Department of Pharmacology, Therapeutics and Toxicology , Lagos, Nigeria
,
Abdullahi A. Adejare
2   Department of Physiology, College of Medicine of the University of Lagos, Lagos, Nigeria
,
Abiodun F. Shodipe
1   Department of Pharmacology, Therapeutics and Toxicology , Lagos, Nigeria
,
Oludaisi A. Oduniyi
,
Khadijah B. Ismail-Badmus
,
Ibrahim A. Oreagba
1   Department of Pharmacology, Therapeutics and Toxicology , Lagos, Nigeria
› Author Affiliations
Further Information

Publication History

received 22 February 2018

accepted 03 May 2018

Publication Date:
26 June 2018 (online)

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Abstract

Background Cisplatin is an anti-cancer drug that causes nephrotoxicity and oxidative stress. Extracts of Nigella sativa is nephroprotective. Vitamin E is also a potent antioxidant. This study sought to determine a possible synergistic effect of administering the two agents prior to cisplatin use on nephrotoxicity and oxidative stress.

Methods 48 male Wistar rats were randomly divided into 6 groups of 8 rats each. Group I served as the control. Group II received cisplatin without any treatment for 6 days. Groups III, IV, V and VI received 100 mg/kg Nigella sativa (NS), 200 mg/kg NS, 100 mg/kg Vitamin E and 200 mg/kg NS+100 mg/kg Vitamin E respectively for 5 days prior to 6 days administration of cisplatin. On the last day of the experiment, all the animals were sacrificed and serum samples collected for analysis.

Results Cisplatin administration caused a significant increase in creatinine level (p<0.01), urea level (p<0.01), sodium concentration and malondialdehyde level (p<0.001). Pre-administration with NS caused a significant reduction in creatinine level (p<0.001), urea level (p<0.001), sodium concentration (p<0.001) and malondialdehyde (p<0.01) level. Pre-administration with vitamin E caused a significant reduction in creatinine level (p<0.001), urea level (p<0.01), sodium concentration (p<0.001) and malondialdehyde level. They both also caused a significant increase in superoxide dismutase, reduced glutathione and catalase (CAT) levels. The combination of NS and vitamin E however did not show significant synergistic effects.

Conclusion These results suggest that even though pre-administration of the two agents protect against renal toxicity and oxidative stress, the effects are however not collaborative.

Key words

Cisplatin - nigella sativa - oxidative stress - renal toxicity - vitamin E
 

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