Abstract
The aim of this work was to evaluate the in vitro performance of nebulized nanosuspension
formulation when nebulized using ultrasonic nebulizer. The present investigation deals
with successful formulation of Beclomethasone dipropionate loaded HPMCP nanospheres
prepared by solvent evaporation technique using PEG 400 as a stabilizer. Beclomethasone
dipropionate is a water insoluble drug molecule was encapsulated in HPMCP nanospheres
to have pH dependent solubility at basic pH for targeted drug delivery in lung and
studied for in vitro cytotoxicity and immediate release capability. The synthesized
nanospheres were characterized through drug excipient compatibility, surface topography;
mean particle size , zeta potential, PDI, entrapment efficiency and drug loading,
in vitro diffusion, aerodynamic, in vitro cytotoxicity and stability studies. The
mean particle size and PDI of the optimized batch (F1) had 197.6±0.40 nm and 0.324
±0.35, respectively. The % entrapment efficiency and % drug loading was found to be
86.56±1.32 and 8.30±0.27, respectively. The optimized batch F1 showed % cumulative
drug release 94.77±0.24 at 1 h. The formulation showed cell viability up to 91.28%.
It can be concluded that, Beclomethasone dipropionate loaded HPMCP nanospheres was
found to be safe, stable with significant increase in solubility and bypass the liver.
Key words
Drug delivery - Anti-asthma / COPD drugs - Pulmonary & respiratory pharmacology
