Drug Res (Stuttg) 2018; 68(09): 504-513
DOI: 10.1055/a-0573-8966
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Monodisperse Rattle-Structured Gold Nanorod-Mesoporous Silica Nanoparticles Core–Shell as Sulforaphane Carrier and its Sustained-Release Property

Hamidreza Kheiri Manjili
1   Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
,
Leila Ma’mani
2   Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran
,
Hossein Naderi-Manesh
3   Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
› Author Affiliations
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Publication History

received 27 September 2017

accepted 06 February 2018

Publication Date:
16 April 2018 (online)

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Abstract

Sulforaphane (SF) was loaded into the multi-functioned rattle-structured gold nanorod mesoporous silica nanoparticles core-shell to improve its stability and efficacy through its efficient delivery to tumors. The rattle-structured gold nanorod mesoporous silica nanoparticles (rattle-structured AuNR@mSiO2 core-shell NPs) were obtained by covering the surface of Au NPs with Ag and mSiO2 shell and subsequently selective Ag shell etching strategy. Then the surface of rattle-structured AuNR@mSiO2 NPs was decorated with thiolated polyethylene glycol-FITC and thiolated polyethylene glycol-folic acid to the designed form. The obtained FITC/FA@ [rattle-structured AuNR@mSiO2] NPs was characterized by different techniques including energy dispersive X-ray spectroscopy (EDX), scanning and transmission electron microscopy (SEM & TEM), UV-visible spectrophotometer and dynamic light scattering (DLS). The FITC/FA@ [rattle-structured AuNR@mSiO2] NPs has an average diameter around ~33 nm, which increases to ~38 nm after the loading of sulforaphane. The amount of the loaded drug was ~ 2.8×10-4 mol of SF per gram of FITC/FA@ [rattle-structured AuNR@mSiO2] NPs. The rattle-structured AuNR@mSiO2 and FITC/FA@ [rattle-structured AuNR@mSiO2] NPs showed little inherent cytotoxicity, whereas the SF loaded FITC/FA@ [rattle-structured AuNR@mSiO2] NPs was highly cytotoxic in the case of MCF-7 cell line. Finally, Fluorescence microscopy and flow cytometry were used to demonstrate that the nanoparticles could be accumulated in specific regions and SF loaded FITC/FA@ [Fe3O4@Au] NPs efficiently induce apoptosis in MCF-7 cell line [Graphical Abstract].

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Graphical Abstract

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