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Homeopathy 2014; 103(04): 275-284
DOI: 10.1016/j.homp.2014.08.002
Original Paper
Copyright © The Faculty of Homeopathy 2014

Modulation of inflammation response to murine cutaneous Leishmaniosis by homeopathic medicines: Thymulin 5cH

Fabiana Rodrigues de Santana
1   Research Center of Universidade Paulista, São Paulo, Brazil
2   Laboratory of Veterinary Pathology, Universidade de Santo Amaro, Brazil
,
Cidéli de Paula Coelho
1   Research Center of Universidade Paulista, São Paulo, Brazil
2   Laboratory of Veterinary Pathology, Universidade de Santo Amaro, Brazil
,
Thayná Neves Cardoso
1   Research Center of Universidade Paulista, São Paulo, Brazil
,
Márcia Dalastra Laurenti
3   Faculdade de Medicina da Universidade de São Paulo, Brazil
,
Elizabeth Cristina Perez Hurtado
1   Research Center of Universidade Paulista, São Paulo, Brazil
,
Leoni Villano Bonamin
1   Research Center of Universidade Paulista, São Paulo, Brazil
2   Laboratory of Veterinary Pathology, Universidade de Santo Amaro, Brazil
› Author Affiliations
Subject Editor:
Further Information

Publication History

Received07 April 2014
revised16 July 2014

accepted26 August 2014

Publication Date:
19 December 2017 (online)

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Background: In previous studies, we observed that thymulin 5cH could modulate BCG (Bacillus Calmette-Guerin) induced chronic inflammation by increasing peritoneal B1 stem cells differentiation into phagocytes and improving phagocytosis efficiency.

Methods: We used the same protocol to study the effects of thymulin 5cH in the experimental murine Leishmaniasis, in order to elucidate some aspects of the parasite–host relation under this homeopathic treatment. Male Balb/c mice were orally treated with thymulin 5cH or vehicle during 60 days, after the subcutaneous inoculation of 2 × 106 units of Leishmania (L.) amazonensis into the footpad. Washied inflammatory cell suspension from peritoneal cavity, spleen, local lymph node and infected subcutaneous tissue were harvested after 2 and 60 days from infection to quantify the inflammation cells by flow cytometry and histometry methods.

Results: After a transitory increase of peritoneal T reg cells, treated mice presented, chronically, increase in the peritoneal and spleen B1 cells percentage (p = 0.0001) in relation to other cell types; more organized and exuberant inflammation response in the infection site, and decrease in the number of parasites per field inside the primary lesion (p = 0.05). No difference was seen in local lymph node histology.

Conclusions: Thymulin 5cH is able to improve B1 cell activation and Leishmania (L) amazonensis phagocytosis efficiency in mice, similarly to that observed previously in BCG experimental infection.

Keywords

Leishmania (L.) amazonensis - Thymulin - Homeopathy - Murine leishmaniasis
 

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