Intracoronary β2 receptor activation induces dynamic local t-PA release in the pig

Barbro Österlund; Christina Jern; Helene Seeman-Lodding; Göran Johansson; Sören Häggmark; Michael Broomé; Björn Biber

doi:10.1160/TH02-09-0013

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2003; 90(05): 796-802
DOI: 10.1160/TH02-09-0013

Blood Coagulation, Fibrinolysis and Cellular Haemostasis

Schattauer GmbH

Intracoronary β2 receptor activation induces dynamic local t-PA release in the pig

Barbro Österlund

¹Surgical and Perioperative Science, Anaesthesiology and Intensive care, Umeå University, Umeå, Sweden

,

Christina Jern

²Clinical Experimental Research Laboratory

³Institute of Clinical Neuroscience and

,

Helene Seeman-Lodding

⁴Anaesthesiology and Intensive Care, Sahlgrenska University Hospital, Göteborg, Sweden

,

Göran Johansson

¹Surgical and Perioperative Science, Anaesthesiology and Intensive care, Umeå University, Umeå, Sweden

,

Sören Häggmark

¹Surgical and Perioperative Science, Anaesthesiology and Intensive care, Umeå University, Umeå, Sweden

,

Michael Broomé

¹Surgical and Perioperative Science, Anaesthesiology and Intensive care, Umeå University, Umeå, Sweden

,

Björn Biber

¹Surgical and Perioperative Science, Anaesthesiology and Intensive care, Umeå University, Umeå, Sweden

› Author Affiliations

Abstract

Summary

To investigate β₂-adrenergic agonist-mediated effects on coronary fluxes of local fibrinolytic factors, healthy anaesthetised and instrumented pigs (n=10) were studied during infusion of isoprenaline (IPR) into the left main coronary artery. Coronary net fluxes of total t-PA antigen, active t-PA and total PAI-1 antigen were determined at baseline and at 3, 5, 7 and 10 minutes of IPR infusion. During IPR, net release of total t-PA increased in a biphasic pattern with transiently high levels at 3 (+440 %) and 7 minutes (+620%) and returned towards baseline at 10 minutes. Net coronary release of active t-PA increased with maximum levels at 3 minutes (+50%). Baseline coronary net flux of total PAI –1 showed a decrease which was most pronounced at 10 minutes. To conclude, a fast β₂agonist-mediated local release of t-PA into the coronary vasculature was demonstrated. For total t-PA, this response was characterised by a biphasic release profile.

Keywords

Tissue-type plasminogen activator - plasminogen activator inhibitor - porcine - coronary circulation - β₂-adrenergic agonism

Full Text

References

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