Molecular amplification of parvovirus B19 DNA in an amniotic fluid sample was used
to confirm the etiologic agent of a case with fetal hydrops. The polymerase chain
reaction (PCR) method used is easy and fast to perform. We have a 2-year experience
on its use in clinical parvovirus B19 diagnosis. The results are available within
1 day of sampling. In addition to strict laboratory standards, the use of two different
sets of PCR primers and probes confirms positive findings. Positive parvovirus PCR
on amniotic fluid confirms fetal parvovirus infection in cases of fetal nonimmune
hydrops. Intrauterine transfusions may be necessary in cases with severe fetal anemia.
Parvovirus B19 infection - polymerase chain reaction - fetal nonimmune hydrops
