Pharmacopsychiatry 1998; 31(5): 193-198
DOI: 10.1055/s-2007-979326
Original Paper

© Georg Thieme Verlag Stuttgart · New York

Active [3H]-Dopamine Uptake Displayed by Native Lymphocyte Suspensions is Mainly Due to Contaminating Platelets

K. Krieger, A. Klimke, U. Henning
  • Department of Psychiatry, University of Düsseldorf, Germany
Further Information

Publication History

Publication Date:
20 April 2007 (online)

Abstract

Kinetic and pharmacologic properties of specific [3H]-dopamine uptake by native human lymphocytes were investigated. Our results suggest that uptake of [3H]-dopamine measured with lymphocytes after separation over Ficoll-Paque™ or Percoll™ is mainly caused by platelets which are always part of freshly prepared lymphocyte suspensions. The investigations were extended to well-defined cell lines in order to compare the pharmacological properties of native and immortalized cells regarding the uptake of [3H]-dopamine without any influence of contaminating cells such as platelets. Using the human neuroblastoma cell line IMR32 we demonstrate a GBR-12909 and cocaine-sensitive specific uptake of dopamine, whereas dopamine uptake in platelets is performed by an imipramine-sensitive serotonin transporter. Blood-derived stable cell lines (MOLT-3 and EBV-transformed B-lymphocytes) exhibited no [3H]-dopamine uptake. The view that specific [3H]-dopamine uptake on native human lymphocytes is mainly caused by platelets and not specific for lymphocytes is supported by the finding that homogenous B- and T-lymphoblastoids (MOLT-3 and EBV-transformed B-lymphocytes) exhibited no comparable uptake.

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