Flow Cytometric Determination of Genome Size in some Old World Lupinus Species (Fabaceae)

Abstract

Genome size determinations using propidium iodide flow cytometry were conducted in Lupinus anatolicus, L. pilosus, L. luteus, L. hispanicus ssp. hispanicus and L. hispanicus ssp. hispanicus × L. luteus (a complex hybridization product of L. hispanicus ssp. hispanicus and L. luteus). Using Clycine max “Ceresia” (1C = 1.134 pg) and an accession of L. pilosus (1C = 0.649 pg) as internal standards the following 1C values were obtained: L. anatolicus: 0.606 pg; L. pilosus: 0.620-0.678 pg; L. luteus: 1.173 pg; L. hispanicus: 1.043 pg; L. hispanicus ssp. hispanicus × L. luteus: 1.036 pg. Lupinus anatolicus is a newly described taxon with smooth seeds whose genome is approximately as small as the smallest genomes measured in the related L. pilosus, a taxon with rough seeds. The genomes in the related smooth-seeded taxa L. luteus, L. hispanicus and L. hispanicus ssp. hispanicus × L. luteus are considerably larger. There is a 1.13-fold difference between L. luteus (crop) and L. hispanicus (wild). The genome size of L. hispanicus ssp. hispanicus × L. luteus, however, is not intermediate, it is close to L. hispanicus.

Key words

Lupinus anatolicus - L. pilosus - L. luteus - L. hispanicus - L. hispanicus ssp. hispanicus × L. luteus - genome size variation - flow cytometry