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DOI: 10.1055/s-2007-978492
© Georg Thieme Verlag Stuttgart · New York
Cultivar-Specific Expression of the Jasmonate-Induced Protein of 23 kDa (JIP-23) Occurs in Hordeum vulgare L. by Jasmonates but not During Seed Germination
Publication History
1998
1998
Publication Date:
19 April 2007 (online)
Abstract
Treatment of barley leaf segments with jasmonic acid methyl ester (JM) leads to the accumulation of a set of newly formed abundant proteins. Among them, the most abundant protein exhibits a molecular mass of 23 kDa (JIP-23). Here, data are presented on the occurrence and expression of the JIP23 genes in different cultivars of Hordeum vulgare. Southern blot analysis of 80 cultivars revealed the occurrence of 2 to 4 genes coding for JIP-23 in all cultivars. By means of Northern blot and immunoblot analysis it is shown that some cultivars lack the expression of jip-23 upon treatment of primary leaves with JM as well as upon stress performed by incubation with 1 M sorbitol solution. During germination, however, all tested cultivars exhibited developmental expression of jip-23. The results are discussed in terms of possible functions of JIP-23 in barley.
Key words
Hordeum vulgare - cultivar-specific expression - jasmonate-induced protein - germination - stress by sorbitol treatment