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Plant Biol (Stuttg) 1999; 1(1): 68-75
DOI: 10.1055/s-2007-978490
Original Papers

© Georg Thieme Verlag Stuttgart · New York

Development of Vacuoles and Vacuolar H+-ATPase Activity under Extremely High CO2 Conditions in Chlorococcum littorale Cells

T. Sasaki1 , Natalia A. Pronina1 , 2 , M. Maeshima3 , I. Iwasaki1 , N. Kurano1 , S. Miyachi4
  • 1Marine Biotechnology Institute, Kamaishi Laboratories, Kamaishi City, Iwate, Japan
  • 2Plant Physiology Institute, Russian Academy of Sciences, Moscow, Russia
  • 3Laboratory of Biochemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan
  • 4Marine Biotechnology Institute, Head Office, Bunkyo-ku, Tokyo, Japan
Further Information

Publication History

1998

1998

Publication Date:
19 April 2007 (online)

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Abstract

The number and cross-sectional area of vacuoles in Chlorococcum littorale cells visualized with a differential interference fluorescence microscope increased after their transfer from air to 40% CO2. An immunological observation indicated that the level of subunit B of vacuolar H+-ATPase also increased under 40% CO2 conditions. The activity of nitrate-sensitive ATPase associated with the vacuolar membrane was 2-fold higher in 40% CO2-grown cells than in air-grown cells. The effects of inhibitors on the ATPase activity confirmed that these activities were derived from vacuolar-type H+-ATPase. These results suggest that vacuole development associated with that of vacuolar H+-ATPase occurred during the acclimatization of C. littorale cells to extremely high CO2 conditions.

Key words

Chlorococcum littorale - extremely high CO2 conditions - vacuolar H+-ATPase - vacuole