Endoscopy 2007; 39: E317-E318
DOI: 10.1055/s-2007-966798
Unusual cases and technical notes

© Georg Thieme Verlag KG Stuttgart · New York

Endocytoscopy assists in the intraoperative diagnosis of carcinoma in a patient with chronic pancreatitis

G.  V.  Rao1 , R.  Pradeep1 , M.  J.  Mansard1 , C.  Ramji1 , R.  Banerjee1 , D.  Nageshwar Reddy1
  • 1Department of Gastroenterology, Asian Institute of Gastroenterology, Hyderabad, Andhra Pradesh, India
Further Information

Publication History

Publication Date:
08 January 2008 (online)

The endocytoscopy system has been used successfully for direct histological imaging of the gastrointestinal mucosa during endoscopy [1] [2] [3]. However, there are no published reports on the intraoperative use of the endocytoscope. This is the first report describing the intraoperatiave use of the endocytoscope for the detection of carcinoma in a patient with chronic pancreatitis.

A 44-year-old man with longstanding chronic pancreatitis presented with recurrent episodes of abdominal pain and significant weight loss. His CA19 - 9 level was raised at 392 U/mL (normal range 0 - 25 U/mL). Computed tomography showed changes typical of chronic pancreatitis, but no evidence of tumor. Elective pancreaticojejunostomy was planned because there were multiple strictures in the pancreatic duct. Intraoperatively, the head of pancreas was bulky but there was no palpable mass. The pancreatic duct was opened and showed a long stricture with ulcerations in the head region.

It was decided that endocytoscopic evaluation might be useful in order to assess the type and extent of the lesion. The opened pancreatic duct was sprayed with sterilized methylene blue dye ([Fig. 1]). The body region of the pancreatic duct was examined first in order to familiarize ourselves with the normal cytoanatomy of the duct, and then we looked at the suspicious area in the head region, which showed clusters of tumor cells with hyperchromatic nuclei and a high nuclear-cytoplasmic ratio ([Fig. 2]). This pattern was found only in the head of the pancreas. A biopsy of this tissue was sent for frozen section examination and subsequently for conventional histopathological examination, which showed malignancy, confirming the endocytoscopy findings ([Fig. 3]).

Fig. 1 Intraoperative photograph of the head and body of the pancreas, showing spraying of the opened pancreatic duct with methylene blue dye.

Fig. 2 Intraoperative cytoscopic images of the pancreatic duct in the pancreatic body/tail region (a) and in the pancreatic head (b).

Fig. 3 Conventional histopathological examination of biopsy tissue from the pancreatic head region confirmed malignancy.

In conclusion, endocytoscopy assisted in making a diagnosis of carcinoma on a background of chronic pancreatitis, by visualizing variation in cell size, disorder of polarity, and deformity of the nuclei. This optical biopsy technique could be useful for the immediate diagnosis of mitotic lesions, definition of the extent of these lesions, and assessment of the negativity of resection margins during surgery.



D. Nageshwar Reddy, MD

Asian Institute of Gastroenterology

6-3-661 Somajiguda

Hyderabad 500 082

Andhra Pradesh


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