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Plant Biol (Stuttg) 2007; 9(4): 526-533
DOI: 10.1055/s-2007-964934
Research Paper

Georg Thieme Verlag Stuttgart KG · New York

Methylation-Sensitive Amplification Polymorphism in Date Palms (Phoenix dactylifera L.) and their Off-Shoots

J.-G. Fang1 , C. T. Chao2
  • 1Horticultural College, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China
  • 2Department of Botany and Plant Sciences, University of California-Riverside, 900 University Av., Riverside, CA 92521-0124, USA
Further Information

Publication History

Received: June 6, 2006

Accepted: December 5, 2006

Publication Date:
20 July 2007 (online)

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Abstract

DNA methylation plays an important role in the regulation of gene expression in eukaryotes. In this study, the extent and patterns of DNA methylation were assessed in date palm mother-plants and their off-shoots using the methylation-sensitive amplified polymorphism (MSAP) technique. Three types of bands were generated using 12 pairs of primers. Type I were present in both EcoR I + Hpa II and EcoR I + Msp I lanes; type II were present in EcoR I + Hpa II lanes, but not in EcoR I + Msp I lanes; and type III bands were present in EcoR I + Msp I lanes, but not in EcoR I + Hpa II lanes. The total numbers of these three types of bands were 782, 55, and 34, respectively. Among these three types of bands, the polymorphic bands were, respectively, 37, 10, and 0. The distribution of polymorphic bands among mother-plants and off-shoots suggests the methylation variation was present in both the mother-plants and off-shoots. Forty- four out of these 47 polymorphic bands show clear difference between mother-plant and off-shoots: 38 were present only in off-shoots and 6 in both mother-plants and off-shoots. Compared to methylation status in mother-plants, the methylation variation during off-shoot growth of date palm can be characterized as a process involving primarily de-methylation. Hypomethylation of DNA in off-shoots, compared with mother-plants, reflects the marked expression of this molecular feature, which may be related to gene expression during off-shoot development. The methylation or de-methylation status of specific loci in the mother-plants and their off-shoots were probably random events.

Key words

Date palm - cytosine methylation - isoschizomers - off-shoots.

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J.-G. Fang

Horticultural College
Nanjing Agricultural University

1 Weigang

Nanjing 210095

China

Email: fanggg@njau.edu.cn

Editor: M. Koornneef