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Horm Metab Res 1985; 17(9): 448-450
DOI: 10.1055/s-2007-1013573
ORIGINALS
Basic
© Georg Thieme Verlag, Stuttgart · New York

Stimulatory Effect of Epidermal Growth Factor on Prostaglandin E2 Production in Mouse Fibrosarcoma Cell Line (HSDM1 C1)

Y. Hirata, M. Uchihashi1 , H. Nakashima1 , T. Fujita1 , S. Matsukura2
  • Hypertension-Endocrine Division, National Cardiovascular Center, Osaka, Japan
  • 1Department of Medicine, Kobe University, School of Medicine, Kobe, Japan
  • 2Department of Medicine, Miyazaki Medical College, Miyazaki, Japan
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Publikationsverlauf

1984

1984

Publikationsdatum:
14. März 2008 (online)

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Summary

Using HSDM1 C1 cell line derived from the mouse fibrosarcoma which synthesizes and secretes prostaglandin (PG)E2, specific binding sites for epidermal growth factor (EGF), a potent growth stimulator of many tissues, and its effect on PGE2 production by cultured tumor cells were studied.

HSDM1 C1 cell line possessed specific, high-affinity receptors for EGF: Kd (5.5 × 10-10 M) and binding capacity (17,650 sites/cell). EGF significantly stimulated PGE2 production in HSDM1 C1 line cultured in serum-free medium for 24 h in a dose-dependent manner; a 2.5-fold increase over control was induced by as little as 0.1 ng/ml and the maximal effect (3.5-fold increase) by 1 ng/ml. Its stimulatory effect on PGE2 production was completely blocked by indomethacin, an inhibitor of PG biosynthesis.

These data suggest that EGF may be involved in modulation of synthesis and/or secretion of PGE2, a potent bone-resorbing factor, by the tumors which may partly contribute to hypercalcemia in certain types of neoplasms.

Key-Words:

EGF - PGE 2 - Mouse Fibrosarcoma Cell Line - Bone-Resorbing Factor