Characterization of Neurotrophic Activity in the Silicone-Chamber Model for Nerve Regeneration

Nils Danielsen; Silvio Varon

doi:10.1055/s-2007-1006537

Journal of Reconstructive Microsurgery, Inhaltsverzeichnis

J Reconstr Microsurg 1995; 11(3): 231-235
DOI: 10.1055/s-2007-1006537

ORIGINAL ARTICLE

Characterization of Neurotrophic Activity in the Silicone-Chamber Model for Nerve Regeneration

Nils Danielsen, Silvio Varon

Departments of Experimental Research and Hand Surgery, Malmö University Hospital, University of Lund, Malmö, Sweden, and Department of Biology, University of California, San Diego, La Jolla, California

Abstract

ABSTRACT

In the present study, the authors reevaluated the temporal course and properties of neurotrophic activities present in the fluid accumulating in the silicone-chamber model for nerve regeneration. The fluid collected from silicone chambers was tested in four different dissociated neuronal cell cultures. Furthermore, the activity of the chamber fluid was examined, using a cell blot technique. There was one major peak of neurotrophic activity and this activity peaked early, about 3 to 6 hr after nerve injury. Results also indicate that the chamber fluid contains at least two types of neurotrophic activities, namely nerve growth factor and ciliary neurotrophic factor.

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