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J Reconstr Microsurg 2006; 22(5): 375-384
DOI: 10.1055/s-2006-946716
Copyright © 2006 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA.

Chimerism after Vascularized Limb Versus Bone Marrow Transplantation

Michael Cohen1 , Thalachallour Mohanakumar2 , Susan E. Mackinnon1 , Thomas H. Tung1
  • 1Division of Plastic and Reconstructive Surgery, Washington University School of Medicine, St. Louis, Missouri
  • 2Section of Transplantation, Department of Surgery, Washington University School of Medicine, St. Louis, Missouri
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Publikationsverlauf

Accepted: March 13, 2006

Publikationsdatum:
17. Juli 2006 (online)

    Lizenzen und Reprints

ABSTRACT

This study used quantitative PCR in the murine model to compare the ability of a limb allograft vs. a comparable dose of marrow suspension to induce chimerism. Female C57Bl/6 mice received a vascularized hindlimb allograft, a comparable dose of 5 × 106 donor bone marrow cells, or a standard dose (20 × 106) of marrow suspension from male Balb/c donors. All recipients were treated with a regimen based on CD40 costimulation blockade and T cell depletion. Y chromosome-specific quantitative PCR was used to measure chimerism. Most recipients of limb allografts demonstrated low levels of chimerism after 1 week (3/4) and 1 month (3/4). Most recipients of 5 × 106 marrow cells had low levels of chimerism at 1 week (4/6) and only 1/5 after 1 month. All recipients of 20 × 106 cells except one demonstrated either low or high levels of chimerism after 1 week (5/5) and 1 month (5/6). The marrow component of a limb allograft is thus more effective at inducing microchimerism compared to a comparable dose of bone marrow suspension.

KEYWORDS

Limb transplantation - costimulation blockade - chimerism - quantitative PCR - bone marrow transplantation

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Thomas H TungM.D. 

Division of Plastic and Reconstructive Surgery, Washington University School of Medicine

660 S. Euclid Avenue, Suite 5401, St. Louis, MO 63110

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