Lipopolysaccharides of Pectobacterium atrosepticum and Pseudomonas corrugata Induce Different Defence Response Patterns in Tobacco, Tomato, and Potato

Abstract

Lipopolysaccharides (LPS), ubiquitous cell surface components of Gram-negative bacteria, are directly implicated in plant/pathogen interactions. However, their perception by the plant, the subsequent signal transduction in both compatible and incompatible interactions, as well as the defence reactions induced in compatible interactions are as yet poorly understood. We focused on biochemical and physiological reactions induced in cell suspensions of three Solanaceae species (tobacco, tomato, and potato) by purified lipopolysaccharides from Pectobacterium atrosepticum (Pa), a pathogen of potato, and Pseudomonas corrugata (Psc), a pathogen of tomato. LPS _Pa and LPS _Psc caused a significant acidification of potato, tomato, and tobacco extracellular media, whereas laminarin (a linear β‐1,3 oligosaccharide elicitor) induced an alkalinisation in tobacco and tomato, but not in potato cell suspensions. None of the two LPS induced the formation of active oxygen species in any of the hosts, while laminarin induced H₂O₂ production in cells of tobacco but not of tomato and potato. In tomato cells, LPS _Pa and LPS _Psc induced a strong but transitory stimulation of lipoxygenase activity, whereas laminarin induced a stable or slightly increasing LOX activity over the first 24 h of contact. In tobacco, LOX activity was not triggered by either LPS, but significantly increased following treatment with laminarin. In potato, neither LPS nor laminarin induced LOX activity, in contrast with concentrated culture filtrate of Phytophthora infestans (CCF). These results demonstrate that LPS, as well as laminarin, are perceived in different ways by Solanaceae species, and possibly cultivars. They also suggest that defence responses modulated by LPS depend on plant genotypes rather than on the type of interaction.

Key words

Extracellular pH - lipopolysaccharide - lipoxygenase - Nicotiana tabacum - reactive oxygen species - Solanaceae - Solanum lycopersicum - Solanum tuberosum cvs. Bintje and Kerpondy

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Editor: C. M. J. Pieterse