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Plant Biol (Stuttg) 2006; 8(4): 494-502
DOI: 10.1055/s-2006-923800
Research Paper

Georg Thieme Verlag Stuttgart KG · New York

At Daybreak - Reproductive Biology and Isolating Mechanisms of Cirrhaea dependens (Orchidaceae)

E. R. Pansarin1 , V. Bittrich1 , M. C. E. Amaral1
  • 1Departamento de Botânica, Instituto de Biologia, Universidade Estadual de Campinas, Caixa Postal 6109, Campinas, São Paulo 13083-970, Brazil
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Publikationsverlauf

Received: September 9, 2005

Accepted: December 9, 2005

Publikationsdatum:
11. Mai 2006 (online)

   Lizenzen und Reprints

Abstract

Floral biology, reproduction, pollinator specificity, and fruit set of Cirrhaea dependens were recorded in forest areas of Southeastern Brazil. Cirrhaea dependens is a lithophytic or epiphytic herb occurring very sparsely below dense canopies. Nearly all the flowers of a single plant open simultaneously before dawn. They are short-lasting and offer floral fragrances as rewards, which are collected by male euglossine bees. Observations carried out in mesophytic forests at Serra do Japi revealed that Euglossa viridis is their principal pollinator, with Eufriesea violacea a sporadic co-pollinator. Visitation started soon after flower opening, and attractiveness remained high for about 2 h, decreasing abruptly at sunrise. Flower anthesis with subsequent fragrance release seems to be correlated with bee attraction. Observations using chemical baits were carried out at Serra do Japi, and in the Atlantic Rain Forest of Picinguaba. Three euglossine species were captured with pollinaria of C. dependens in Picinguaba, whereas only E. violacea was attracted in Serra do Japi. In Picinguaba, C. dependens occurs sympatrically with C. saccata and C. loddigesii. Each species attracted different pollinators. The specificity and resulting reproductive isolation are due to the production of different odours by each orchid species. Cirrhaea dependens is self-compatible but pollinator-dependent. The reproductive success was low and appears to result from a combination of factors discussed here, such as the production of short-lived flowers, presence of floral mechanisms avoiding self-pollination, non-synchronization of flower phases among plants, and populations with few and sparsely distributed individuals.

Key words

Cirrhaea - Euglossini - Orchidaceae - pollination - reproductive biology - reproductive success - Stanhopeinae.

References

  • 1 Ackerman J. D.. Specificity and mutual dependency of the orchid-euglossine bee interaction.  Biological Journal of the Linnean Society. (1983 a);  20 301-314
    PubMedGoogle Scholar
  • 2 Ackerman J. D.. Euglossine bee pollination of the orchid Cochleanthes lipscombiae: a food source mimic.  American Journal of Botany. (1983 b);  70 830-834
    CrossrefPubMedGoogle Scholar
  • 3 Ackerman J. D.. Diversity and seasonality of male euglossine bees (Hymenoptera: Apidae) in Central Panamá.  Ecology. (1983 c);  64 274-283
    CrossrefPubMedGoogle Scholar
  • 4 Ackerman J. D.. Coping with the epiphytic existence: pollination strategies.  Selbyana. (1986);  9 52-60
    PubMedGoogle Scholar
  • 5 Ackerman J. D.. Geographic and seasonal variation in fragrance choices and preferences of male euglossine bees.  Biotropica. (1989);  21 340-347
    PubMedGoogle Scholar
  • 6 Armbruster W. S., McCormick K. D.. Diel foraging patterns of male euglossine bees: ecological causes and evolutionary response by plants.  Biotropica. (1990);  22 160-171
    CrossrefPubMedGoogle Scholar
  • 7 Armbruster W. S., Berg E. E.. Thermal ecology of male euglossine bees in a tropical wet forest: fragrance foraging in relation to operative temperature.  Biotropica. (1994);  26 50-60
    CrossrefPubMedGoogle Scholar
  • 8 Becker P., Moure J. S., Peralta F. J.. More about euglossine bees in amazonian forest fragments.  Biotropica. (1991);  23 586-591
    CrossrefPubMedGoogle Scholar
  • 9 Braga P. I.. Atração de abelhas polinizadoras de Orchidaceae com auxílio de iscas-odores na campina, campinarana e floresta tropical úmida da região de Manaus.  Ciência e Cultura. (1976 a);  28 767-773
    PubMedGoogle Scholar
  • 10 Braga P. I.. Estudos da flora orquidológica do Amazonas. I. Descrição e observação da biologia floral de Stanhopea candida Barb. Rodr.  Acta Amazonica. (1976 b);  6 433-438
    PubMedGoogle Scholar
  • 11 Casey T. M., May M. L., Morgan K. R.. Flight energetics of euglossine bees in relation to morphology and wing stroke frequency.  The Journal of Experimental Biology. (1985);  116 271-289
    PubMedGoogle Scholar
  • 12 Curry K. J., McDowell L. M., Judd W. S., Stern W. L.. Osmophores, floral features, and systematics of Stanhopea (Orchidaceae).  American Journal of Botany. (1991);  78 610-623
    CrossrefPubMedGoogle Scholar
  • 13 Dodson C. H.. The role of chemical attractants in orchid pollination. Chambers, K. L., ed. Biochemical Coevolution. Oregon; Oregon State Univesity Press (1970): 83-107
    Google Scholar
  • 14 Dodson C. H., Dressler R. L., Hills H. G., Adams R. M., Williams N. H.. Biologically active compounds in orchid fragrances.  Science. (1969);  164 1243-1249
    PubMedGoogle Scholar
  • 15 Dressler R. L.. Pollination by euglossine bees.  Evolution. (1968);  22 202-210
    PubMedGoogle Scholar
  • 16 Dressler R. L.. How to study orchid pollination without any orchids. Senghas, K., ed. Proceedings of the Eighth World Conference. German Orchid Society , Frankfurt, West Germany. (1976): 534-537
    Google Scholar
  • 17 Dressler R. L.. The Orchids: Natural History and Classification. Massachusetts; Harvard University Press (1981)
    Google Scholar
  • 18 Dressler R. L.. Biology of the orchid bees (Euglossini).  Annual Review of Ecology and Systematics. (1982);  13 373-394
    PubMedGoogle Scholar
  • 19 Dressler R. L.. Phylogeny and Classification of the Orchid Family. Cambridge; Cambridge University Press (1993)
    Google Scholar
  • 20 Gerlach G.. Sievekingia y Soterosanthus: parientes, pero diferentes. Sievekingia and Soterosanthus: related by different.  Orquideología. (1999);  21 214-227
    PubMedGoogle Scholar
  • 21 Gerlach G., Schill R.. Composition of orchid scents attracting euglossine bees.  Botanica Acta. (1991);  104 379-391
    PubMedGoogle Scholar
  • 22 Heinrich B.. Energetics of pollination.  Annual Review of Ecology and Systematics. (1975);  6 139-170
    CrossrefPubMedGoogle Scholar
  • 23 Heinrich B.. Bumblebee Economics. Massachusetts; Harvard University Press (1979)
    Google Scholar
  • 24 Hills H. G., Williams N. H., Dodson C. H.. Identification of some orchid fragrance.  American Orchid Society Bulletin. (1968);  37 967-971
    PubMedGoogle Scholar
  • 25 Hills H. G., Williams N. H., Dodson C. H.. Floral fragrances and isolating mechanisms in the genus Catasetum (Orchidaceae).  Biotropica. (1972);  4 61-76
    CrossrefPubMedGoogle Scholar
  • 26 Hoehne F. C.. Contribuição para o conhecimento do gênero Catasetum, especialmente hermaphroditismo e trimorphismo das suas flores.  Boletim de Agricultura do Estado de São Paulo. (1933);  1 133-196
    PubMedGoogle Scholar
  • 27 Hoehne F. C.. Iconografia de Orquidaceas do Brasil. São Paulo; S. A. Indústrias “Graphicars F. Lanzara” (1942)
    Google Scholar
  • 28 Inouye D. W.. Flight temperatures of male euglossine bees (Hymenoptera: Apidae: Euglossini).  Journal of the Kansas Entomological Society. (1975);  48 366-377
    PubMedGoogle Scholar
  • 29 Janzen D. H., DeVries P. J., Higgins M. L., Kimsey L. S.. Seasonal and site variation in Costa Rican euglossine bees at chemical baits in lowland deciduous and evergreen forests.  Ecology. (1982);  63 66-74
    CrossrefPubMedGoogle Scholar
  • 30 Kaiser R.. The Scent of the Orchids. Olfactory and Chemical Investigations. Amsterdam; Elsevier (1993)
    Google Scholar
  • 31 Kimsey L. S.. Systematics of Bees of the Genus Eufriesea (Hymenoptera, Apidae). Berkeley; University of California Press (1982)
    Google Scholar
  • 32 Martini P., Schlindwein C., Montenegro A.. Pollination, flower longevity, and reproductive biology of Gongora quinquenervis Ruíz and Pavón (Orchidaceae) in an Atlantic forest fragment of Pernambuco, Brazil.  Plant Biology. (2003);  5 495-503
    Artikel in Thieme ConnectPubMedGoogle Scholar
  • 33 May M. L., Casey T. M.. Thermoregulation and heat exchange in euglossine bees.  Physiological Zoology. (1983);  56 541-551
    PubMedGoogle Scholar
  • 34 Miller D., Warren R.. Orquídeas do Alto da Serra. 2nd edition. Rio de Janeiro; Salamandra (1996)
    Google Scholar
  • 35 Morrison A.. Cirrhaea. Pridgeon, A., ed. The Illustrated Encyclopedia of Orchids. Oregon; Timber Press (1997): 63
    Google Scholar
  • 36 Murren C. J.. Effects of habitat fragmentation on pollination: pollinators, pollinia viability and reproductive success.  Journal of Ecology. (2002);  90 100-107
    CrossrefPubMedGoogle Scholar
  • 37 Neiland M. R. M., Wilcock C. C.. Fruit set, nectar reward, and rarity in the Orchidaceae.  American Journal of Botany. (1998);  85 1657-1671
    PubMedGoogle Scholar
  • 38 Nimer E.. Climatologia da região sudeste do Brasil.  Revista Brasileira de Geografia. (1977);  34 3-48
    PubMedGoogle Scholar
  • 39 van der Pijl L., Dodson C. H.. Orchid Flowers: Their Pollination and Evolution. Coral Gables; University of Miami Press (1966)
    Google Scholar
  • 40 Pinto H. S.. O clima da Serra do Japi. Morellato, L. P. C., ed. História Natural da Serra do Japi. Campinas; Editora da Unicamp/Fapesp (1992): 30-38
    Google Scholar
  • 41 Powell A. H., Powell G. V. N.. Population dynamics of male euglossine bees in Amazonian forest fragments.  Biotropica. (1987);  19 176-179
    CrossrefPubMedGoogle Scholar
  • 42 Ricklefs R. E., Adams R. M., Dressler R. L.. Species diversity of Euglossa in Panama.  Ecology. (1969);  50 713-716
    CrossrefPubMedGoogle Scholar
  • 43 Roubik D. W., Ackerman J. D.. Long-term ecology of euglossine orchid-bees (Apidae: Euglossini) in Panama.  Oecologia. (1987);  73 321-333
    CrossrefPubMedGoogle Scholar
  • 44 Sakai W. S.. Simple method for differential staining of paraffin embedded plant material using toluidine blue O.  Stain Technology. (1973);  43 247-249
    PubMedGoogle Scholar
  • 45 Singer R. B., Sazima M.. Abelhas Euglossini como polinizadoras de orquídeas na região de Picinguaba, São Paulo, Brasil. Barros, F. and Kerbauy, G. B., eds. Orquidologia Sul-Americana: Uma Compilação Científica. São Paulo; Secretaria do Meio Ambiente (2004): 175-187
    Google Scholar
  • 46 Whitten W. M., Williams N. H., Armbruster W. S., Battiste M. A., Strekowski L., Lindquist N.. Carvone oxide: an example of convergent evolution in euglossine pollinated plants.  Systematic Botany. (1986);  11 222-228
    CrossrefPubMedGoogle Scholar
  • 47 Williams N. H.. The biology of orchids and euglossine bees. Arditti, J., ed. Orchid Biology and Perspectives. Ithaca; Cornell University Press (1982): 119-171
    Google Scholar
  • 48 Williams N. H., Dodson C. H.. Selective attraction of male euglossine bees to orchid floral fragrances and its importance in long distance pollen flow.  Evolution. (1972);  28 84-95
    PubMedGoogle Scholar
  • 49 Williams N. H., Atwood J. T., Dodson C. H.. Floral fragrance analysis in Anguloa, Lycaste, and Mendoncella (Orchidaceae).  Selbyana. (1981);  5 291-295
    PubMedGoogle Scholar
  • 50 Williams N. H., Whitten W. M.. Orchid floral fragrances and male euglossine bees: methods and advances in the last sesquidecade.  Biological Bulletin. (1983);  164 355-395
    PubMedGoogle Scholar
  • 51 Zimmerman J. K., Roubik D. W., Ackerman J. D.. Asynchronous phenologies of a neotropical orchid and its euglossine bee pollinator.  Ecology. (1989);  70 1192-1195
    CrossrefPubMedGoogle Scholar

E. R. Pansarin

Departamento de Botânica
Instituto de Biologia
Universidade Estadual de Campinas

Caixa Postal 6109

Campinas, São Paulo 13083-970

Brazil

eMail: epansarin@uol.com.br

Editor: S. Renner

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