Endoscopy 2004; 36 - 54
DOI: 10.1055/s-2004-825036

SELDI-MS Protein Profiling Differentiates Ovarian From Colon Cell Lines

AM Lennon 1, D Stuart 1, AC Tan 1, KS Sheahan 1, DP O'Donoghue 1, B Brown 1
  • 1Department of Medical Oncology, Beatson Institute, Glasgow University. Department of Biostatistics, Glasgow University, Glasgow, Scotland. The Centre for Colorectal Disease, St. Vincent's University Hospital, Dublin, Ireland

Introduction Surface enhanced laser desorption ionisation mass spectrometry (SELDI-MS) is a technique which has been used to study proteomic patterns. The aim of this study was to determine the sensitivity of SELDI-MS in differentiating two different cell lines.

Methods 198 protein peaks were generated from 13 cell line extracts. Ciphergen Biomarker Wizard was used to identify significant peaks and an in-house decision analysis tree was used to differentiate the two sample types.

Results We were able to differentiate ovarian from colon cell lines using only one peptide marker with a sensitivity and specificity of 84.6% and 100% for colorectal and 100% and 84.6% for ovarian cell lines.

Discussion This initial study demonstrates the potential of SELDI-MS to differentiate two different cell lines with a high level of sensitivity and specificity. The number of cell lines used was small and needs to be increased to validate these results. If this result can be replicated in tissue samples it may be of considerable benefit as conventional histology and immunohistochemical assays are of limited value in differentiating colorectal from ovarian metastatic deposits.