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Thorac Cardiovasc Surg 2004; 52(5): 305-311
DOI: 10.1055/s-2004-821153
Special Report

© Georg Thieme Verlag KG Stuttgart · New York

A Murine Model of Ischemic Cardiomyopathy Induced by Repetitive Ischemia and Reperfusion[*]

O. Dewald1 , 2 , N. G. Frangogiannis1 , M. P. Zoerlein1 , G. D. Duerr1 , G. Taffet1 , L. H. Michael1 , A. Welz2 , M. L. Entman1
  • 1Section of Cardiovascular Sciences and DeBakey Heart Center, Baylor College of Medicine, and The Methodist Hospital, Houston, TX, USA
  • 2Department of Cardiac Surgery, Rheinische-Friedrich-Wilhelms University of Bonn, Germany
Further Information

Publication History

Received May 23, 2004

Publication Date:
06 October 2004 (online)

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Abstract

Background: Repetitive brief myocardial ischemia has been implicated in the pathogenesis of the ventricular dysfunction associated with ischemic cardiomyopathy and myocardial hibernation. In this study we examine the effects of repetitive ischemia and reperfusion (I/R) on murine myocardium. Methods: C57/BL6 mice underwent daily 15 min left anterior descending coronary occlusions followed by reperfusion. After 3, 5, 7, 14, 21 and 28 days, echocardiographic studies were performed, and hearts of I/R and sham-operated animals were processed for histological examination. Results: Histological studies showed no evidence of myocardial necrosis in the ischemic region. Quantitative assessment of collagen revealed a marked persistent interstitial deposition of collagen after seven days I/R in the anterior left ventricular wall (sham 4.6 ± 2.0 %, I/R 21.5 ± 6.5 %, p < 0.05). Echocardiographic studies showed persistent regional anterior wall dysfunction in I/R animals. Histological evaluation showed absence of neovessel formation. After discontinuation of the I/R protocol, fibrosis and regional ventricular dysfunction decreased within 60 days. Conclusions: Repetitive brief murine myocardial I/R induces reversible fibrotic remodeling and ventricular dysfunction, without myocardial infarction and necrosis, and may play a role in the pathogenesis of ischemic cardiomyopathy and myocardial hibernation.

Key words

Ischemia - reperfusion - myocardium - hibernation

1 Ernst Derra Award at the 4th Joint Meeting of German, Austrian, and Swiss Society for Thoracic and Cardiovascular Surgery, Hamburg, February 15 - 18, 2004

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1 Ernst Derra Award at the 4th Joint Meeting of German, Austrian, and Swiss Society for Thoracic and Cardiovascular Surgery, Hamburg, February 15 - 18, 2004

Oliver Dewald

Department of Cardiac Surgery, University Clinical Center Bonn

Sigmund-Freud-Str. 25

53105 Bonn

Germany

Phone: + 492282875109

Fax: + 49 22 82 87 41 95

Email: Oliver.Dewald@ukb.uni-bonn.de

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