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J Reconstr Microsurg 2004; 20(2): 183-192
DOI: 10.1055/s-2004-820776
Copyright © 2004 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA

Improved Early Muscle Recovery Using FK506 in a Rat Nerve-Repair Model

Mustafa Asim Aydin1 , 2 , Melanie G. Urbanchek2 , 3 , William M. Kuzon2 , 3 , 4  Jr 
  • 1Department of Plastic Surgery, Suleyman Demirel University, Isparta, Turkey
  • 2Division of Plastic Surgery, University of Michigan, Ann Arbor, MI
  • 3Department of Veterans Affairs Medical Center, Ann Arbor, MI
  • 4Institute of Gerontology, University of Michigan, Ann Arbor, MI
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Publication History

Publication Date:
09 March 2004 (online)

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The authors hypothesized that FK506 improves maximal muscle force (P0) recovery during early muscle reinnervation. Rat peroneal nerve was unilaterally cut and repaired. Rats received subcutaneous injections of vehicle (controls) or FK506 at 1 mg/kg (FK506-1MG) or 5 mg/kg (FK506-5MG) for 3 weeks. Extensor digitorum longus (EDL) isometric muscle P0 was measured bilaterally 4 weeks postoperatively. FK506 treatment adversely affected body mass. With body mass as a covariate, the P0 for denervated-reinnervated muscle in the FK506-5MG group was statistically significantly higher by 34 percent and 32 percent, compared with controls and FK506-1MG groups. There was no group difference for the contralateral, nerve-intact EDL muscle P0, when loss of body mass was considered. NCAM immunohistologic data showed significantly better reinnervation of the denervated muscle in a dose-dependent fashion. Thus, FK506 treatment at high dose improves early recovery of force in denervated-reinnervated muscle, following nerve repair, when loss of body mass is considered.

KEYWORDS

Tacrolimus - denervation - muscle

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Melanie G UrbanchekPh.D. 

University of Michigan, Section of Plastic and Reconstructive Surgery

2130 Taubman Health Care Center

1500 E. Medical Center Drive

Ann Arbor, MI 48109-0340

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