Molecular Cytogenetics of Xeranthemum L. and Related Genera (Asteraceae, Cardueae)

Abstract

Seven representatives of the genera Amphoricarpus, Chardinia, Siebera, and Xeranthemum, all of them closely related as demonstraded by molecular phylogeny, have been studied from a cytogenetic perspective. Morphometrical karyotype parameters were calculated and idiograms obtained. Fluorochrome banding was performed with chromomycin A₃ to identify GC-rich regions in the chromosomes. Fluorescence in situ hybridization allowed us to locate the sites of 18S-5.8S-26S and 5S rDNA. Silver nitrate staining was used to count the number of nucleoli and to detect the active nucleolar organizing regions. Systematic and evolutionary issues are addressed in the light of these data.

Key words

Amphoricarpus - Chardinia - Siebera - Compositae - fluorochrome banding - fluorescence in situ hybridization - genome organization - nucleolar organizing regions.

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Section Editor: F. Salamini