Mtha1, a Plasma Membrane H+-ATPase Gene from Medicago truncatula, Shows Arbuscule-Specific Induced Expression in Mycorrhizal Tissue

F. Krajinski; B. Hause; V. Gianinazzi-Pearson; P. Franken

doi:10.1055/s-2002-37407

Plant Biology, Inhaltsverzeichnis

Plant Biol (Stuttg) 2002; 4(6): 754-761
DOI: 10.1055/s-2002-37407

Original Paper

Georg Thieme Verlag Stuttgart ·New York

Mtha1, a Plasma Membrane H⁺-ATPase Gene from Medicago truncatula, Shows Arbuscule-Specific Induced Expression in Mycorrhizal Tissue

F. Krajinski ^1,2 , B. Hause ³ , V. Gianinazzi-Pearson ⁴ , P. Franken ^1,5

¹ Max-Planck-Institute for Terrestrial Microbiology, and Laboratory for Microbiology, Philipps-University, Karl-von-Frisch-Strasse, 35043 Marburg, Germany
² Present address: LG Molecular Genetics, University of Hannover, Herrenhäuser Strasse 2, 30419 Hannover, Germany
³ Institute of Plant Biochemistry, Department of Secondary Metabolism, P.O. Box 110432, 06018 Halle, Germany
⁴ UMR 1088 INRA/University of Burgundy, BBCE-IPM, INRA-CMSE, BP 86510, 21065 Dijon Cedex, France
⁵ Institute for Vegetable and Ornamental Plants, Grossbeeren, Germany

Abstract

Transport processes between plant and fungal cells are key elements in arbuscular mycorrhiza (AM), where H⁺-ATPases are considered to be involved in active uptake of nutrients from the symbiotic interface. Genes encoding H⁺-ATPases were identified in the genome of Medicago truncatula and three cDNA fragments of the H⁺-ATPase gene family (Mtha1 - 3) were obtained by RT-PCR using RNA from M. truncatula mycorrhizal roots as template. While Mtha2 and Mtha3 appeared to be constitutively expressed in roots and unaffected by AM development, transcripts of Mtha1 could only be detected in AM tissues and not in controls. Further analyses by RT-PCR revealed that Mtha1 transcripts are not detectable in shoots and phosphate availability did not affect RNA accumulation of the gene. Localization of transcripts by in situ hybridization on AM tissues showed that Mtha1 RNA accumulates only in cells containing fungal arbuscules. This is the first report of arbuscule-specific induced expression of a plant H⁺-ATPase gene in mycorrhizal tissues.

Key words

Arbuscular mycorrhiza - in situ hybridization - plant nutrition - RNA accumulation - symbiosis

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P. Franken

Institute for Vegetable and Ornamental Plants

Theodor-Echtermeyer-Weg 1
14979 Grossbeeren
Germany

eMail: franken@igzev.de

Section Editor: J. Cullimore