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Plant Biol (Stuttg) 2002; 4(4): 484-491
DOI: 10.1055/s-2002-34133
Original Paper
Georg Thieme Verlag Stuttgart ·New York

Comparative Study of the Ploidy Series Bromus sterilis, B. diandrus and B. rigidus (Poaceae) Based on Chromosome Numbers, Morphology and Isozymes

T. Oja 1 , H. Laarmann 2
  • 1 Botany Department, Institute of Zoology and Botany, Estonian Agricultural University, Tartu, Estonia
  • 2 Institute of Botany, University of Regensburg, Germany
Further Information

Publication History

Received: December 3, 2001

Accepted: June 24, 2002

Publication Date:
18 September 2002 (online)

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Abstract

Chromosome numbers, morphological characters and isozymes of seven enzymes were studied to assess relationships between species of the Bromus diandrus-rigidus polyploid complex and the closely related species, B. sterilis. The four different cytotypes detected, 2n = 14, 28, 42 and 56, could be divided into two species: B. sterilis (2n = 14, 28) and B. diandrus (2n = 42, 56) by morphological features and isozymes. The shape of the scar of rachilla segments in the floret proved to be a suitable character for distinguishing the two species, but not the chromosomal races within species. The tetraploid shared homozygous isozyme phenotypes with diploid B. sterilis at all loci, except one, suggesting that it is autopolyploid. No diagnostic isozymes could be found to distinguish between hexa- and octoploid cytotypes in the B. diandrus-rigidus complex.

Abbreviations

AAT: aspartate aminotransferase

ANOVA: analysis of variance

MDH: malate dehydrogenase

MLILs: multilocus isozyme lineages

PGD: phosphogluconate dehydrogenase

PGI: phosphoglucoisomerase

PRX: peroxidase

SOD: superoxide dismutase

SKD: shikimate dehydrogenase

UPGMA: unweighted pair-group average method

Key words

Bromus - cytotaxonomy - morphological variation - isozyme diversity

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T. Oja

Botany Department
Institute of Zoology and Botany
Estonian Agricultural University, Estonia

181 Riia str.
51014 Tartu
Estonia

Email: tanja@zbi.ee

Section Editor: F. Salamini