Investigations on Net CO₂ Assimilation, Transpiration and Root Growth of Fagus sylvatica Infested with Four Different Phytophthora Species

Abstract

In this contribution, we compare the influence of four different Phytophthora species on root development, net CO₂ assimilation and transpiration of beech seedlings and saplings. Some few days after inoculation, photosynthesis and transpiration of seedlings infected with either P. citricola or P. cambivora were strongly reduced. In parallel, about 60 % of their root systems was destroyed compared to control plants. Three weeks after infection, all seedlings were dead, showing severe wilt symptoms on leaves. Remarkably, P. syringae and P. undulata infected seedlings and older beech plants did not differ from controls regarding photosynthesis and transpiration, although the root systems were damaged. However, a significant influence on net CO₂ assimilation and transpiration of P. citricola infected beech saplings was visible after bud break in the following year. Some days before plants started to wilt, photosynthesis and transpiration were reduced to almost zero. Water use efficiency data (WUE) clearly indicated that infected plants suffered from severe drought.

Abbreviations

WUE: water use efficiency

PPFD: photosynthetic photon flux density

Key words

Fagus sylvatica - Phytophthora - root rot - photosynthesis - transpiration - water use efficiency (WUE)

References

• 01 Baker,  B.,, Zambryski,  P.,, Staskawicz,  B.,, and Dinesh-Kumar,  S. P.. (1997);  Signalling in plant-microbe interactions.  Science. 276 726-733
• 02 Cahill,  D. M., and McComb,  J. A.. (1992);  A comparison of changes in phenylalanine ammonium-lyase activity, lignin and phenolic synthesis in the roots of Eucalyptus calophylla (field resistant) and E. marginata (susceptible) when infected with Phytophthora cinnamomi. .  Physiol. Mol. Plant Pathol.. 40 315-332
• 03 Cahill,  D. M.,, Weste,  G. M.,, and Grant,  B. R.. (1986);  Changes in cytokinin concentrations in xylem exudates following infection of Eucalyptus marginata Donn ex Sm with Phytophthora cinnamomi Rands.  Plant Physiol.. 81 1103-1109
• 04 Dawson,  P., and Weste,  G.. (1984);  Impact of root infection by Phytophthora cinnamomi on the water relations of two Eucalyptus species that differ in susceptibility.  Phytopathol.. 74 486-490
• 05 Day,  W. R.. (1938);  Root-rot of sweet chestnut and beech caused by species of Phytophthora. Cause and symptoms of disease: its relation to soil conditions.  Forestry. 12 101-116
• 06 Duniway,  J. M.. (1977);  Changes in resistance to water transport in safflower during the development of Phytophthora root rot.  Phytopathol.. 67 331-337
• 07 Duniway,  J. M.. (1975);  Water relations in safflower during wilting induced by Phytophthora root rot.  Phytopathol.. 65 886-891
• 08 Ebel,  J., and Scheel,  D.. (1997) Signals in host parasite interactions. The Mycota V Part A: Plant Relationships. Carroll, G. C. and Tudzynski, P., eds. Berlin, Heidelberg; Springer-Verlag p. 85-105
• 09 Grams,  T. E. E.,, Anegg,  S.,, Häberle,  K.-H.,, Langebartels,  C.,, and Matyssek,  R.. (1999);  Interactions of chronic exposure to elevated CO₂ and O₃ levels in the photosynthetic light and dark reactions of European beech (Fagus sylvatica). .  New Phytol.. 144 95-107
• 10 Günthardt-Georg,  M. S.,, McQuattie,  C. J.,, Scheidegger,  C.,, Rhiner,  C.,, and Matyssek,  R.. (1997);  Ozone-induced cytochemical and ultrastructural changes in leaf mesophyll cell walls.  Can. J. For. Res.. 27 453-463
• 11 Hartmann,  G., and Blank,  R.. (1998);  Buchensterben auf zeitweise nassen Standorten unter Beteiligung von Phytophthora-Wurzelfäule.  Forst und Holz. 53 187-193
• 12 Heiser,  I.,, Fromm,  J.,, Giefing,  M.,, Koehl,  J.,, Jung,  T.,, and Oßwald,  W. F.. (1999);  Investigations on the action of Phytophthora quercina, P. citricola and P. gonapodyides toxins on tobacco plants.  Plant Physiol. Biochem.. 37 73-81
• 13 Jung,  T., and Blaschke,  H.. (1996);  Phytophthora root-rot in declining forest trees.  Phyton (Austria). 36 95-102
• 14 Jung,  T.,, Neumann,  P.,, and Blaschke,  H.. (1996);  Isolation, identification and pathogenicity of Phytophthora species from declining oak stands.  Eur. J. For. Path.. 26 253-272
• 15 Jung,  T.,, Blaschke,  H.,, and Oßwald,  W. F.. (2000);  Involvement of soil-borne Phytophthora species in Central European oak decline and the influence of site factors on the disease.  Plant Pathol.. 29 169-188
• 16 Luque,  J.,, Cohen,  M.,, Save,  R.,, Biel,  C.,, and Alvarez,  I. F.. (1999);  Effects of three fungal pathogens on water relations, chlorophyll fluorescence and growth of Quercus suber L.  Ann. For. Sci.. 56 19-26
• 17 Matyssek,  R.,, Günthardt-Georg,  M. S.,, Keller,  M. S.,, and Schneidegger,  C. H.. (1991);  Impairment of gas exchange in birch leaves (Betula pendula) caused by low ozone concentrations.  Trees. 5 5-13
• 18 Matyssek,  R., and Innes,  J. L.. (1999);  Ozone - a risk factor for trees and forests in Europe?.  Water, Air and Soil Pollution. 116 199-226
• 19 Maurer,  S.,, Matyssek,  R.,, Günthardt-Georg,  M. S.,, Landolt,  W.,, and Einig,  W.. (1997);  Nutrition and ozone sensitivity of birch (Betula pendula), I. Responses at leaf level.  Trees. 12 1-10
• 20 McManus,  P. S., and Ewers,  F. W.. (1990);  The effect of Cryphonectria parasitica on water relations of American chestnut.  Physiol. Mol. Plant Pathol.. 36 461-470
• 21 Nürnberger,  T.. (1999);  Signal perception in plant pathogen defense.  CMLS Cell. Mol. Life Sci.. 55 167-182
• 22 Okey,  E. N.,, Duncan,  E. J.,, Sirju-Charran,  G.,, and Sreenivasan,  T. N.. (1997);  Phytophthora canker resistance in cacao: Role of peroxidase, polyphenoloxidase and phenylalanine ammonium lyase.  J. Phytopathol.. 145 195-299
• 23 Oßwald,  W. F.. (2000) Biochemical defense reactions of woody plants infested with Phytophthora or Pythium fungi. Proceedings of the Cost Action G4. Thessaloniki, Greece; Multidisciplinary Chestnut Research pp. 1-15
• 24 Ploetz,  R. C., and Schaffer,  B.. (1989);  Effects of flooding and Phytophthora root rot on net gas exchange and growth of Avocado.  Phytopathol.. 79 204-208
• 25 Polle,  A.,, Matyssek,  R.,, Günthardt-Georg,  M. S.,, and Maurer,  S.. (1999) Defense strategies against ozone in trees: the role of nutrition. Environmental pollution and plant responses. Agrawal, S. B. and Agrawal M., eds. New York, Boca Raton; Levis Publishers pp. 223-245
• 26 Sandermann,  H., Jr.. (1996);  Ozone and plant health.  Annu. Rev. Phytopathol.. 34 347-366
• 27 Sandermann,  H., Jr.. (2000);  Ozone/biotic disease interactions: molecular biomarkers as a new experimental tool.  Environmental Pollution. 108 327-332
• 28 Schramm,  R. J., and Wolf,  F. T.. (1954);  The transpiration of black shank infected tobacco.  J. Elisha Mitchell Sci.. 70 255-261
• 29 Schulze,  E.-D., and Hall,  A. E.. (1982) Stomatal responses, water loss, and nutrient relations in contrasting environment. Encyclopedia of plant ecology 12 B, Physiological plant ecology II. Lange, O. L., Nobel, P. S., Osmond, C. B., and Ziegler, H., eds. Berlin, Heidelberg, New York; Springer Verlag pp. 182-230
• 30 Sterne,  R. E.,, Kaufmann,  M. R.,, and Zentmyer,  G. A.. (1978);  Effects of Phytophthora root rot on water relations of Avocado: Interpretation with a water transport model.  Phytopathol.. 68 595-602
• 31 Tsao,  P. H., and Guy,  S. O.. (1977);  Inhibition of Mortierella and Pythium in a Phytophthora-isolation medium containing hymexazol.  Phytopathol.. 67 796-801
• 32 Werres,  S.. (1995);  Influence of the Phytophthora isolate and the seed source on the development of beech (Fagus sylvatica) seedling blight.  Eur. J. For. Pathol.. 25 381-390
• 33 Wolf,  F. T., and Wolf,  F. A.. (1954);  Toxicity as a factor in tobacco black shank.  J. Elisha Mitchell Sci.. 70 244-255
• 34 Wolf,  F. T.. (1933);  The pathology of tobacco black shank.  Phytopathology. 23 605-612

Section Editor: M. Riederer