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Plant Biol (Stuttg) 2000; 2(5): 571-578
DOI: 10.1055/s-2000-7471
Original Paper
Georg Thieme Verlag Stuttgart ·New York

Evaluation of Morphological Variation in the Lichen Diploschistes ocellatus (Ascomycota, Ostropales): Evidence from Nuclear rDNA ITS Sequence Data

M. P. Martín 1 , K. Winka 2 , X. Llimona 3 , and H. T. Lumbsch 4
  • 1 Real Jardín Botánico, C.S.I.C., Madrid, Spain
  • 2 Department of Ecology and Environmental Science, Umeå University, Umeå, Sweden
  • 3 Departament de Biologia Vegetal, Universitat de Barcelona, Barcelona, Spain
  • 4 Botanisches Institut, Universität Essen, Essen, Germany
Further Information

Publication History

March 2, 2000

July 10, 2000

Publication Date:
31 December 2000 (online)

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Abstract

A morphologically distinct population (var. almeriensis) of the lichen species Diploschistes ocellatus from southern Spain is examined using morphological and molecular methods. While the morphological examination revealed some unique features of var. almeriensis, the ITS sequence data did not support a distinction from D. ocellatus s. str. The Kishino-Hasegawa test did not rule out trees distinguishing between the two morphotypes, although spectral analytical data support an inclusion of var. almeriensis in D. ocellatus. The influence of potentially erronously aligned ambiguous sites is evaluated by analysis of different alignments. However, all alignments agree in placing all specimens of D. ocellatus together and, consequently, in the consensus tree D. ocellatus s. lat. forms a monophyletic clade with high bootstrap support.

Key words

Lichens - Ascomycotina - Ostropales - Diploschistes - ribosomal DNA - molecular phylogeny - morphology - species

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H. T. Lumbsch

Botanisches Institut Universität Essen

45117 Essen Germany

Email: lumbsch@uni-essen.de

Section Editor: H. M. Jahns

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