Plant Biol (Stuttg) 2000; 2(4): 403-407
DOI: 10.1055/s-2000-5957
Original Paper
Georg Thieme Verlag Stuttgart ·New York

Variation of Apigenin Quantity in Diploid and Tetraploid Chamomilla recutita (L.) Rauschert

V. Švehlíková, M. Repčák
  • Department of Experimental Botany and Genetics, Faculty of Science, P. J. Šafárik University, Košice, Slovakia
Further Information

Publication History

Febrary 1, 2000

May 16, 2000

Publication Date:
31 December 2000 (online)

Abstract

Apigenin, a pharmacologically important flavonoid of the chamomile plant, was analyzed at two ploidy levels during a three-year period. This flavonoid accumulates in the ligulate florets of the anthodium. Higher percentages of apigenin were found in the ligulate florets of a diploid cultivar, in comparison with tetraploid plants. However, when the total apigenin (mg of compound) in the anthodium was evaluated, tetraploid individuals accumulated significantly more flavonoid. Moreover, in contrast to morphological quantitative characteristics of the anthodium, which varied significantly in different years, apigenin percentage in the ligulate florets was constant and not influenced by environmental conditions. Apigenin content was also found to change during inflorescence ontogeny. It represents the highest percentage of dry mass in young developing florets and anthodia of both cultivars. The total apigenin content of the anthodium, however, increases during flowering, although at later stages apigenin forms only a minor part of ligulate floret and anthodium dry mass.

References

  • 01 Achterrath-Tuckermann,  U.,, Kunde,  R.,, Flaskamp,  E.,, Isaac,  O.,, and Thiemer,  K.. (1980);  Pharmakologische Untersuchungen von Kamillen-Inhaltsstoffen. V. Untersuchungen über die spasmolytische Wirkung von Kamillen-Inhaltsstoffen und von Kamillosan an isolierten Meerschweinchen-Ileum.  Planta Med.. 39 38-50
  • 02 Agullo,  G.,, Gamet-Payrastre,  L.,, Manenti,  S.,, Viala,  C.,, Remesy,  C.,, Chap,  H.,, and Payrastre,  B.. (1997);  Relationship between flavonoid structure and inhibition of phosphatidylinositol-3-kinase: a comparison with tyrosine kinase and protein kinase C inhibition.  Biochem. Pharmacol.. 53 1649-1657
  • 03 Birt,  D. F.,, Mitchell,  D.,, Gold,  B.,, Pour,  P.,, and Pinch,  H. C.. (1997);  Inhibition of ultraviolet light induced skin carcinogenesis in SKH-1 mice by apigenin, a plant flavonoid.  Anticancer Res.. 17 85-91
  • 04 Carle,  R.,, Dölle,  B.,, Müller,  W.,, and Baumeister,  U.. (1993);  Thermospray liquid chromatography/mass spectrometry (TSP LC/MS): Analysis of acetylated apigenin-7-glucosides from Chamomilla recutita. .  Pharmazie. 48 304-306
  • 05 Carle,  R.,, Dölle,  B.,, and Reinhard,  E.. (1989);  A new approach to the production of chamomile extracts.  Planta Med.. 55 540-543
  • 06 Chen,  Z. Y.,, Chan,  P. T.,, Ho,  K. Y.,, Fung,  K. P.,, and Wang,  J.. (1996);  Antioxidant activity of natural flavonoids is governed by number and location of their aromatic hydroxyl groups.  Chem. Phys. Lipids. 79 157-163
  • 07 Cook,  N. C., and Samman,  S.. (1996);  Flavonoids - chemistry, metabolism, cardioprotective effects, and dietary sources.  J. Nutr. Biochem.. 7 66-76
  • 08 Critchfield,  J. W.,, Butera,  S. T.,, and Folks,  T. M.. (1996);  Inhibition of HIV activation in latently infected cells by flavonoid compounds.  AIDS Res. Hum. Retroviruses. 12 39-46
  • 09 Critchfield,  J. W.,, Coligan,  J. E.,, Folks,  T. M.,, and Butera,  S. T.. (1997);  Casein kinase II is a selective target of HIV-1 transcriptional inhibitors.  Proc. Natl. Acad. Sci. USA. 94 6110-6115
  • 10 Della Logia,  R.,, Tubaro,  A.,, Dri,  P.,, Zilli,  C.,, and Del Negro,  P.. (1986) The role of flavonoids in the antiinflamatory activity of Chamomilla recutita. . Plant flavonoids in medicine: biochemical, pharmacological and structure-activity relationship. New York; A.R. Liss pp. 481-484
  • 11 Dölle,  B.,, Carle,  R.,, and Müller,  W.. (1985);  Flavonoidbestimmung in Kamillenextraktpraeparaten.  Dtsch. Apoth. Ztg.. 125 14-19
  • 12 Giles,  D., and Wei,  H.. (1997);  Effect of structurally related flavones/isoflavones on hydrogen peroxide production and oxidative DNA damage in phorbol ester-stimulated HL-60 cells.  Nutr. Cancer. 29 77-82
  • 13 Griesbach,  R. J., and Kamo,  K. K.. (1996);  The effect of induced polyploidy on the flavonols of Petunia “Mitchell”.  Phytochemistry. 42 361-363
  • 14 Ho,  C.,, Ryu,  S. Y.,, Oak,  M. H.,, Cheon,  S. H.,, Yoo,  G. S.,, and Kim,  K. M.. (1998);  Studies of structure activity relationship of flavonoids for the anti-allergic actions.  Arch. Pharm. Res.. 21 478-480
  • 15 Lepley,  D. M.,, Li,  B.,, Birt,  D. F.,, and Pelling,  J. C.. (1996);  The chemopreventive flavonoid apigenin induces G2/M arrest in keratinocytes.  Carcinogenesis. 17 2367-2375
  • 16 Lepley,  D. M., and Pelling,  J. C.. (1997);  Induction of p21/WAF1 and G1 cell-cycle arrest by the chemopreventive agent.  Mol. Carcinog.. 19 74-82
  • 17 Lin,  J. K.,, Chen,  Y. C.,, Huang,  Y. T.,, and Lin-Shiau,  S. Y.. (1997);  Suppression of protein kinase C and nuclear oncogene expression as possible molecular mechanisms of cancer chemoprevention by apigenin and curcumin.  J. Cell Biochem. Suppl.. 28 39-48
  • 18 Middleton,  E., Jr., and Kandaswami,  C.. (1994) The impact of plant flavonoids on mammalian biology: implications for immunity, inflammation and cancer. Flavonoids. Advances in research since 1986. Harborne J. B., ed. London; Chapman and Hall pp. 619-652
  • 19 Redaelli,  C.,, Formentini,  L.,, and Santaniello,  E.. (1980);  Apigenin 7-glucoside and its 2″- and 6″-acetates from ligulate flowers of Matricaria chamomilla. .  Phytochemistry. 19 985-986
  • 20 Redaelli,  C.,, Formentini,  L.,, and Santaniello,  E.. (1981);  Reversed-phased high perfomance liquid chromatography analysis of apigenin and its glucosides in flowers of Matricaria chamomilla and chamomile extracts.  Planta Med.. 42 288-292
  • 21 Redaelli,  C.,, Formentini,  L.,, and Santaniello,  E.. (1982);  Apigenin 7-glucoside diacetates in ligulate flowers of Matricaria chamomilla. .  Phytochemistry. 21 1828-1830
  • 22 Repčák,  M.,, Černaj,  P.,, and Mártonfi,  P.. (1993);  The essential oil content and composition in diploid and tetraploid Chamomilla recutita during the ontogenesis of anthodia.  J. Essent. Oil Res.. 5 297-300
  • 23 Repčák,  M., and Mártonfi,  P.. (1995);  The variability pattern of apigenin glucosides in Chamomilla recutita diploid and tetraploid cultivars.  Pharmazie. 50 696-699
  • 24 Repčák,  M.,, Švehlíková,  V.,, Imrich,  J.,, and Pihlaja,  K.. (1999);  Jaceidin and chrysosplenetin chemotypes of Chamomilla recutita (L.) Rauschert.  Biochem. Syst. Ecol.. 27 727-732
  • 25 Sato,  F.,, Matsukawa,  Y.,, Matsumoto,  K.,, Nishino,  H.,, and Sakai,  T.. (1994);  Apigenin induces morphological differentiation and G2-M arrest in rat neuronal Cells.  Biochem. Biophys. Res. Commun.. 204 578-584
  • 26 Shirley,  B. W.. (1998);  Flavonoids in seeds and grains: physiological function, agronomic importance and the genetics of biosynthesis.  Seed Sci. Res.. 8 415-422
  • 27 Wang,  H. K.,, Xia,  Y.,, Yang,  Z. Y., Natschke,  S. L.,, and Lee,  K. H.. (1998);  Recent advances in the discovery and development of flavonoids and their analogues as antitumor and anti-HIV agents.  Adv. Exp. Med. Biol.. 439 191-225
  • 28 Williams,  C. A., and Harborne,  J. B.. (1994) Flavone and flavonol glycosides. Flavonoids. Advances in research since 1986. Harborne J. B., ed. London; Chapman and Hall pp. 337-386
  • 29 Yin,  F.,, Giuliano,  A. E.,, and van Herle,  A. J.. (1999);  Signal pathways involved in apigenin inhibition of growth and induction of apoptosis of human anaplastic thyroid cancer cells.  Anticancer Res.. 19 4297-4303
  • 30 Yokozawa,  T.,, Chen,  C. P.,, Dong,  E.,, Tanaka,  T.,, Nonaka,  G. I.,, and Nishioka,  I.. (1998);  Study on the inhibitory effect of tannins and flavonoids against the 1,1-diphenyl-2-picrylhydrazyl radical.  Biochem. Pharmacol.. 56 213-222

V. Švehlíková

Department of Experimental Botany and Genetics Faculty of Science P. J. Šafárik University

Mánesova 23 04154 Košice Slovakia

Email: svehlik@kosice.upjs.sk

Section Editor: U. Lüttge

    >