Exp Clin Endocrinol Diabetes 2018; 126(03): 187-193
DOI: 10.1055/s-0043-118749
Article
© Georg Thieme Verlag KG Stuttgart · New York

Effects of Aldosterone on Chemerin Expression And Secretion in 3T3-L1 Adipocytes

Junhua Ma*
1   Department of Endocrinology, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai 200135, China
,
Fei Sun*
1   Department of Endocrinology, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai 200135, China
,
Jingnan Wang*
1   Department of Endocrinology, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai 200135, China
,
He Jiang
2   Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China
,
Jiancan Lu
1   Department of Endocrinology, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai 200135, China
,
Xing Wang
1   Department of Endocrinology, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai 200135, China
,
Jichen Zhang
1   Department of Endocrinology, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai 200135, China
,
Chao Shi
1   Department of Endocrinology, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai 200135, China
,
Wen You
1   Department of Endocrinology, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai 200135, China
,
Xiangqi Li
1   Department of Endocrinology, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai 200135, China
,
Hongling Zhu
1   Department of Endocrinology, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai 200135, China
,
Mingjun Gu
1   Department of Endocrinology, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai 200135, China
› Author Affiliations
Further Information

Publication History

received 11 June 2017
revised 03 August 2017

accepted 22 August 2017

Publication Date:
20 September 2017 (online)

Abstract

Aldosterone plays a pivotal role in the pathogenesis of metabolic syndrome and cardiovascular disease; however, the underlying mechanisms have not been clarified. Chemerin has been characterized as an adipokine with crucial roles in obesity-associated disorders and cardiovascular homeostasis. The aim of the present study was to investigate the direct effects of aldosterone on chemerin expression and secretion in 3T3-L1 adipocytes and to identify the potential signalling pathways involved. Chemerin mRNA levels were measured using real-time PCR, whereas the levels of secreted chemerin in the culture media were determined using ELISA. Treatment with aldosterone induced time- and dose-dependent increases of chemerin gene expression and protein secretion, and effect that was mediated through the mineralocorticoid receptor. Signalling studies suggested that the NF-κB pathway is involved in aldosterone-induced chemerin expression. Taken together, our data demonstrate a direct interaction between aldosterone and chemerin in adipocytes, which may be an underlying mechanism linking aldosterone-associated metabolic abnormalities and cardiovascular disease.

* These authors contributed equally to this work.