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CC BY 4.0 · Eur J Dent 2023; 17(02): 403-410
DOI: 10.1055/s-0042-1748197
Original Article

Histological Examination of Lemongrass Resorbable Dressing on Gingival Healing after Gingivectomy in Rats

Amaliya Amaliya 
1   Departement of Periodontology, Dental Faculty, Universitas Padjadjaran, West Java, Indonesia
,
Indri Budhirahardjo
2   Periodontal Clinic, Indonesian Naval Dental Institute Raden Eddy Martadinata, Central Jakarta
,
Ina Hendiani
1   Departement of Periodontology, Dental Faculty, Universitas Padjadjaran, West Java, Indonesia
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Abstract

Objectives The present work aimed to evaluate the effect of lemongrass extract incorporated in a resorbable periodontal dressing on gingival wound healing microscopically, following gingivectomy in rats.

Materials and Methods Thirty healthy adult male Sprague–Dawley rats were used in this study. Gingivectomy was performed on anterior area of lower jaw in the labial surface of central incisive and, subsequently, wound areas were covered with povidone iodine gel (group P, positive control, n = 10), lemongrass resorbable dressing (group L, n = 10), and a cellulose-based dressing containing myrrh (group M, positive control, n = 10). Histological changes were monitored in days 4, 7, and 14 postsurgery to evaluate fibroblast and collagen deposition as repair stage of healing process.

Statistycal Analysis One-way analysis of variance (ANOVA) followed by Tukey's post hoc for multiple comparisons were employed to measure differences between pairs of means, p-value of 0.05 was considered statistically significant.

Results We observed significant difference repair parameters of the healing process between surgical sites treated with lemongrass periodontal dressing and control groups. Wounds treated with lemongrass dressing had greater fibroblast compared with control groups in 4 and 7 days after surgery (p ≤ 0.05).

Conclusion The results suggest positive potential therapeutic effects for this new formulation of periodontal dressing on acceleration of surgical wound healing that lead to improvement of periodontal treatment consequences following gingivectomy.

Keywords

lemongrass - dressing - wound healing - gingivectomy


Publication History

Article published online:
27 June 2022

© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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  • References

  • 1 Soheilifar S, Bidgoli M, Faradmal J, Soheilifar S. Effect of periodontal dressing on wound healing and patient satisfaction following periodontal flap surgery. J Dent (Tehran) 2015; 12 (02) 151-156
    Google Scholar
  • 2 Löe H, Silness J. Tissue reactions to a new gingivectomy pack. Oral Surg Oral Med Oral Pathol 1961; 14 (11) 1305-1314
    CrossrefPubMedGoogle Scholar
  • 3 Stahl SS, Witkin GJ, Heller A, Brown Jr R. Gingival healing. 3. The effects of periodontal dressings on gingivectomy repair. J Periodontol 1969; 40 (01) 34-37
    CrossrefPubMedGoogle Scholar
  • 4 Goldman HM. Gingivectomy; indications, contraindications, and method. Am J Orthod Oral Surg 1946; 32 (oral surg): 323-326
    CrossrefPubMedGoogle Scholar
  • 5 Asboe-Jörgensen V, Attström R, Lang NP, Löe H. Effect of a chlorhexidine dressing on the healing after periodontal surgery. J Periodontol 1974; 45 (01) 13-17
    CrossrefPubMedGoogle Scholar
  • 6 Breloff JP, Caffesse RG. Effect of Achromycin ointment on healing following periodontal surgery. J Periodontol 1983; 54 (06) 368-372
    CrossrefPubMedGoogle Scholar
  • 7 Slots J, Rams TE. Antibiotics in periodontal therapy: advantages and disadvantages. J Clin Periodontol 1990; 17 (7, pt. 2): 479-493
    CrossrefPubMedGoogle Scholar
  • 8 Pandurić DG, Miličić M, Doblanović K, Sušić M. Refinement in treatment of surgical wounds–Reso-Pac periodontal bandage. Accessed February 12, 2021 at: https://bib.irb.hr/datoteka/578870.ResoPac-Artikel-Panduric-GB-06-kl.pdf
    Google Scholar
  • 9 Skaria BP, Joy PP, Mathew S, Mathew G. Lemongrass. In: Peter KV. ed. Handbook of Herbs and Spices. Philadelphia, PA: Woodhead Publishing; 2006
    Google Scholar
  • 10 Gupta BK, Jain N. Cultivation and utilization of genus Cymbopogon in Indian. Indian Perfumer 1978; 22 (02) 55-68
    Google Scholar
  • 11 Olorunnisola SK, Hammed AM, Simsek S. Biological properties of lemongrass: an overview. Int Food Res J 2014;21(02):
    Google Scholar
  • 12 Balakrishnan B, Paramasivam S, Arulkumar A. Evaluation of the lemongrass plant (Cymbopogon citratus) extracted in different solvents for antioxidant and antibacterial activity against human pathogens. Asian Pac J Trop Dis 2014; 4: S134-S139
    CrossrefGoogle Scholar
  • 13 Kasole R, Martin HD, Kimiywe J. Traditional medicine and its role in the management of diabetes mellitus: “patients' and herbalists' perspectives.”. Evid Based Complement Alternat Med 2019; 2019: 2835691
    CrossrefPubMedGoogle Scholar
  • 14 Nambiar VS, Matela H. Potential functions of lemon grass (Cymbopogon citratus) in health and disease. Int J Pharm Biol Arch 2012; 3 (05) 1035-1043
    Google Scholar
  • 15 Shah G, Shri R, Panchal V, Sharma N, Singh B, Mann AS. Scientific basis for the therapeutic use of Cymbopogon citratus, stapf (Lemon grass). J Adv Pharm Technol Res 2011; 2 (01) 3-8
    CrossrefPubMedGoogle Scholar
  • 16 Naik MI, Fomda BA, Jaykumar E, Bhat JA. Antibacterial activity of lemongrass (Cymbopogon citratus) oil against some selected pathogenic bacterias. Asian Pac J Trop Med 2010; 3 (07) 535-538
    CrossrefGoogle Scholar
  • 17 Vinciguerra LL, Rohr C, Heberlé G. Feasibility study for antiseptic gel formulations incorporating the essential oils Cymbopogon citratus (DC.) Staph. and Caryophyllus aromaticus L. Drug Analytical Res. 2018; 2 (01) 8-12
    CrossrefGoogle Scholar
  • 18 Figueirinha A, Cruz MT, Francisco V, Lopes MC, Batista MT. Anti-inflammatory activity of Cymbopogon citratus leaf infusion in lipopolysaccharide-stimulated dendritic cells: contribution of the polyphenols. J Med Food 2010; 13 (03) 681-690
    CrossrefPubMedGoogle Scholar
  • 19 Oyedele AO, Gbolade AA, Sosan MB, Adewoyin FB, Soyelu OL, Orafidiya OO. Formulation of an effective mosquito-repellent topical product from lemongrass oil. Phytomedicine 2002; 9 (03) 259-262
    CrossrefPubMedGoogle Scholar
  • 20 Wright SC, Maree JE, Sibanyoni M. Treatment of oral thrush in HIV/AIDS patients with lemon juice and lemon grass (Cymbopogon citratus) and gentian violet. Phytomedicine 2009; 16 (2,3): 118-124
    CrossrefPubMedGoogle Scholar
  • 21 Subha DS, Pradeep T. Periodontal therapy with 0.25% lemongrass oil mouthwash in reducing risk of cardiovascular diseases: a 3-arm prospective parallel experimental study. Ethiop J Health Sci 2017; 27 (05) 531-540
    CrossrefPubMedGoogle Scholar
  • 22 Goyal R, Bhat SG, Kamath S. et al. A novel anti-oxidant lemon grass oil mouthwash-a clinical trial. Asian J Exp Biol Sci 2011;2(03):
    Google Scholar
  • 23 Dany SS, Mohanty P, Tangade P, Rajput P, Batra M. Efficacy of 0.25% lemongrass oil mouthwash: a three arm prospective parallel clinical study. J Clin Diagn Res 2015; 9 (10) ZC13-ZC17
    PubMedGoogle Scholar
  • 24 Warad SB, Kolar SS, Kalburgi V, Kalburgi NB. Lemongrass essential oil gel as a local drug delivery agent for the treatment of periodontitis. Anc Sci Life 2013; 32 (04) 205-211
    CrossrefPubMedGoogle Scholar
  • 25 Kilkenny C, Browne W, Cuthill IC, Emerson M, Altman DG. NC3Rs Reporting Guidelines Working Group. Animal research: reporting in vivo experiments: the ARRIVE guidelines. Br J Pharmacol 2010; 160 (07) 1577-1579
    CrossrefPubMedGoogle Scholar
  • 26 Doillon CJ, Silver FH, Olson RM, Kamath CY, Berg RA. Fibroblast and epidermal cell-type I collagen interactions: cell culture and human studies. Scanning Microsc 1988; 2 (02) 985-992
    PubMedGoogle Scholar
  • 27 Darby IA, Hewitson TD. Fibroblast differentiation in wound healing and fibrosis. Int Rev Cytol 2007; 257: 143-179
    CrossrefPubMedGoogle Scholar
  • 28 Rittié L. Cellular mechanisms of skin repair in humans and other mammals. J Cell Commun Signal 2016; 10 (02) 103-120
    CrossrefPubMedGoogle Scholar
  • 29 van Beurden HE, Snoek PA, Von den Hoff JW, Torensma R, Kuijpers-Jagtman AM. Fibroblast subpopulations in intra-oral wound healing. Wound Repair Regen 2003; 11 (01) 55-63
    CrossrefPubMedGoogle Scholar
  • 30 Carlson MA, Longaker MT. The fibroblast-populated collagen matrix as a model of wound healing: a review of the evidence. Wound Repair Regen 2004; 12 (02) 134-147
    CrossrefPubMedGoogle Scholar
  • 31 Sousa F, Machado V, Botelho J, Proença L, Mendes JJ, Alves R. Effect of A-PRF application on palatal wound healing after free gingival graft harvesting: a prospective randomized study. Eur J Dent 2020; 14 (01) 63-69
    Article in Thieme ConnectPubMedGoogle Scholar
  • 32 Primasari A, Sinulingga ER. Differences of wound healing in the buccal part and oral mucosa labial after giving lemongrass extracts (Cymbopogon ciratus). Int J Homeopathy Nat Med. 2019; 5 (01) 36-41
    CrossrefGoogle Scholar
  • 33 Gottardi W. Iodine and iodine compounds. In: Block SS. ed. Disinfection, Sterilization and Preservation. Philadelphia, PA: Lea & Febiger; 1991: 152-166
    Google Scholar
  • 34 Al-Mobeeriek A. Effects of myrrh on intra-oral mucosal wounds compared with tetracycline- and chlorhexidine-based mouthwashes. Clin Cosmet Investig Dent 2011; 3: 53-58
    CrossrefPubMedGoogle Scholar
  • 35 Haffor ASA. Effect of myrrh (Commiphora molmol) on leukocyte levels before and during healing from gastric ulcer or skin injury. J Immunotoxicol 2010; 7 (01) 68-75
    CrossrefPubMedGoogle Scholar
  • 36 Faiga NN, Rachmadi P, Meizarini A. Neovascular pattern in wound healing after zinc oxide and Curcuma longa rhizome extract dressing application. Contemp Clin Dent 2018; 9 (Suppl. 02) S337-S341
    PubMedGoogle Scholar
  • 37 Amaliya A, Muhaimina RK, Susanto A, Sutjiatmo AB. Histological assessment of palatal donor site wound healing after application of Moringa oleifera Lamarck leaf extract in rats. Eur J Dent 2019; 13 (02) 248-254
    Article in Thieme ConnectPubMedGoogle Scholar
  • 38 Kumar MS, Gurunathan D. Lemongrass in dental health. Drug Invent Today. 2019;11(03):
    Google Scholar
  • 39 Rêgo CB, Silva AM, Gonçalves LIM, Paschoal MAB. In vitro antimicrobial activity of essential oil of Cymbopogon citratus (lemon grass) on Streptococcus mutans biofilm. Afr J Microbiol Res 2016; 10 (31) 1224-1228
    CrossrefGoogle Scholar
  • 40 Ambade SV, Deshpande NM. (Antimicrobial and antibiofilm activity of essential oil of Cymbopogon citratus against oral microflora associated with dental plaque. Eur J Med Plants 2019; 1-11
    CrossrefGoogle Scholar
  • 41 Palmer RJ, Darveau R, Lamont RJ, Nyvad B, Teles RP. Human oral Bacterial Biofilms: Composition, Dynamics, and Pathogenesis. In Biofilm Infections. New York, NY: Springer; 2011: 35-68
    Google Scholar
  • 42 Khongkhunthian S, Sookkhee S, Okonogi S. Antimicrobial activities against periodontopathogens of essential oil from lemon grass (Cymbopogon citratus (DC.) Stapf.). Chiang Mai Univ J Nat Sci. 2009; 8: 11-22
    Google Scholar
  • 43 Lakhdar L, Farah A, Bajjou T, Rida S, Bouziane A, Ennibi O. In vitro antibacterial activity of essentials oils from Mentha pulegium, Citrus aurantium and Cymbopogon citratus on Virulent Strains of Aggregatibacter actinomycetemcomitans . Int J Pharmacognosy Phytochem Res. 2015; 6: 1035-1042
    Google Scholar
  • 44 Nasution DL. The Effectivity of Lemongrass (Cymbopogon Citratus) Extract Against Porphyromonas gingivalis ATCC 33277 (IN-VITRO). In International Dental Conference of Sumatera Utara 2017 (IDCSU 2017). 2018; Atlantis Press
    Google Scholar
  • 45 Aiemsaard J, Aiumlamai S, Aromdee C, Taweechaisupapong S, Khunkitti W. The effect of lemongrass oil and its major components on clinical isolate mastitis pathogens and their mechanisms of action on Staphylococcus aureus DMST 4745. Res Vet Sci 2011; 91 (03) e31-e37
    CrossrefPubMedGoogle Scholar
  • 46 Boukhatem MN, Ferhat MA, Kameli A, Saidi F, Kebir HT. Lemon grass (Cymbopogon citratus) essential oil as a potent anti-inflammatory and antifungal drugs. Libyan J Med 2014; 9 (01) 25431
    CrossrefPubMedGoogle Scholar
  • 47 Abe S, Maruyama N, Hayama K. et al. Suppression of tumor necrosis factor-alpha-induced neutrophil adherence responses by essential oils. Mediators Inflamm 2003a; 12 (06) 323-328
    CrossrefPubMedGoogle Scholar
  • 48 Abe S, Sato Y, Inoue S. et al. Anti-Candida albicans activity of essential oils including lemongrass (Cymbopogon citratus) oil and its component, citral [in Japanese]. Nippon Ishinkin Gakkai Zasshi 2003b; 44 (04) 285-291
    CrossrefPubMedGoogle Scholar
  • 49 Cheel J, Theoduloz C, Rodríguez J, Schmeda-Hirschmann G. Free radical scavengers and antioxidants from lemongrass (Cymbopogon citratus (DC.) Stapf.). J Agric Food Chem 2005; 53 (07) 2511-2517
    CrossrefPubMedGoogle Scholar
  • 50 Somparn N, Saenthaweeuk S, Naowaboot J, Thaeomor A, Kukongviriyapan V. Effect of lemongrass water extract supplementation on atherogenic index and antioxidant status in rats. Acta Pharm 2018; 68 (02) 185-197
    CrossrefPubMedGoogle Scholar
  • 51 Kapoor M, Clarkson AN, Sutherland BA, Appleton I. The role of antioxidants in models of inflammation: emphasis on L-arginine and arachidonic acid metabolism. Inflammopharmacology 2005; 12 (5,6): 505-519
    CrossrefPubMedGoogle Scholar