Histological Assessment of Palatal Donor Site Wound Healing after Application of Moringa oleifera Lamarck Leaf Extract in Rats

Abstract

Objective Palatal excisional wound healing in 60 Sprague–Dawley rats was studied for a period of 14 days.

Materials and Methods The wounds were made with a punch biopsy instrument with a diameter of 4 mm. After wounding, the test groups received a topical gel of Moringa oleifera Lamarck 2% or 4% leaf extract, whereas control groups received povidone iodine gel and hydroxypropyl methylcellulose (HPMC 4%). Three rats were killed at 0, 3, 7, 10, and 14 days after wounding from each allocated group. The wounds were assessed at different control times and light microscopy was employed to view serial sections from blocks with magnification.

Results Histological examination revealed that group treated with M. oleifera Lamarck 4% leaf extract showed the highest fibroblast synthesis and collagen deposition.

Conclusion This study suggests that M. oleifera Lamarck leaf extract could be developed as a therapeutic agent for wound healing.

Keywords

open wound - healing - histological assay - Moringa oleifera Lamarck

• References

• 1 Wessel JR, Tatakis DN. Patient outcomes following subepithelial connective tissue graft and free gingival graft procedures. J Periodontol 2008; 79 (03) 425-430
• 2 Del Pizzo M, Modica F, Bethaz N, Priotto P, Romagnoli R. The connective tissue graft: a comparative clinical evaluation of wound healing at the palatal donor site. A preliminary study. J Clin Periodontol 2002; 29 (09) 848-854
• 3 Farnoush A. Techniques for the protection and coverage of the donor sites in free soft tissue grafts. J Periodontol 1978; 49 (08) 403-405
• 4 Nemcovsky CE, Artzi Z, Moses O, Gelernter I. Healing of dehiscence defects at delayed-immediate implant sites primarily closed by a rotated palatal flap following extraction. Int J Oral Maxillofac Implants 2000; 15 (04) 550-558
• 5 Nooh N, Graves DT. Healing is delayed in oral compared to dermal excisional wounds. J Periodontol 2003; 74 (02) 242-246
• 6 Kozlovsky A, Artzi Z, Hirshberg A, Israeli-Tobias C, Reich L. Effect of local antimicrobial agents on excisional palatal wound healing: a clinical and histomorphometric study in rats. J Clin Periodontol 2007; 34 (02) 164-171
• 7 Tenover FC. Mechanisms of antimicrobial resistance in bacteria. Am J Infect Control 2006; 34 (05) (Suppl. 01) S3-S10 discussionS64–S73
• 8 Stewart PS, Costerton JW. Antibiotic resistance of bacteria in biofilms. Lancet 2001; 358 (9276): 135-138
• 9 Kusmana C, Hikmat A. Keanekaragamanhayati flora di Indonesia. Jurnal Pengelolaan Sumberdaya Alamdan Lingkungan. Journal of Natural Resources and Environmental Management 2015; 5 (02) 187-198
• 10 Ebert AW. Promotion of indigenous vegetables in Asia: conservation and use of selected crops in Indonesia, the Philippines, and Taiwan. In: ISHS Acta Horticulturae 918: XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): III International Symposium on Plant Genetic Resources. 2010: 397-403
• 11 Sabastian G, Dahlia L, Mahrizal M. et al. Ethnobotanical use and commercial potential of Moringa oleifera in Indonesia: an underused and under-recognized species. In: ISHS Acta Horticulturae 1158 : I International Symposium on Moringa. 2015: 349-356
• 12 Siddhuraju P, Becker K. Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (. Moringa oleifera. Lam.) leaves. J Agric Food Chem 2003; 51 (08) 2144-2155
• 13 Oluduro AO. Evaluation of antimicrobial properties and nutritional potentials of. Moringa oleifera. Lam. leaf in south-western Nigeria. Malays J Microbiol 2012; 8 (02) 59-67
• 14 Rao PK, Anjana M, Lavanya KD, Madhavi Y, Rao TR. In vitro antibacterial screening of selected medicinal plant extracts against oral pathogens. Asian Journal of Experimental Sciences 2009; 23 (03) 479-485
• 15 Moura MC, Trentin DS, Napoleão TH. et al. Multi-effect of the water-soluble. Moringa oleifera. lectin against. Serratia marcescens. and. Bacillus. sp.: antibacterial, antibiofilm and anti-adhesive properties. J Appl Microbiol 2017; 123 (04) 861-874
• 16 Alsaraf KM, Abd ST, Husain NS. An antimicrobial activity of. Moringa Oleif. era extract in comparison to chlorhexidine gluconate (in vitro study). Journal of Baghdad College of Dentistry 2016; 28 (01) 183-187
• 17 Elgamily H, Moussa A, Elboraey A, El-Sayed H, Al-Moghazy M, Abdalla A. Microbiological assessment of Moringa oleifera extracts and its incorporation in novel dental remedies against some oral pathogens. Open Access Maced. J Med Sci 2016; 4 (04) 585-590
• 18 Sahrakary M, Nazemian V, Aghaloo M. et al. Treatment by. Moringa oleifera. extract can reduce gingival inflammatory cytokines in the rat periodontal model. Physiology and Pharmacology 2017; 21 (02) 102-109
• 19 Kilkenny C, Browne W, Cuthill IC, Emerson M, Altman DG. NC3Rs Reporting Guidelines Working Group. Animal research: reporting in vivo experiments: the ARRIVE guidelines. Br J Pharmacol 2010; 160 (07) 1577-1579
• 20 Oda Y, Kagami H, Ueda M. Accelerating effects of basic fibroblast growth factor on wound healing of rat palatal mucosa. J Oral Maxillofac Surg 2004; 62 (01) 73-80
• 21 Doillon CJ, Silver FH, Olson RM, Kamath CY, Berg RA. Fibroblast and epidermal cell-type I collagen interactions: cell culture and human studies. Scanning Microsc 1988; 2 (02) 985-992
• 22 Hukkeri VI, Nagathan CV, Karadi RV, Patil BS. Antipyretic and wound healing activities of. Moringa oleifera. Lam. in rats. Indian J Pharm Sci 2006; 68 (01) 124
• 23 Lambole V, Kumar U. Effect of. Moringa oleifera. Lam. on normal and dexamethasone suppressed wound healing. Asian Pac J Trop Biomed 2012; 2 (01) S219-S223
• 24 Clark RA. Fibrin and wound healing. Ann N Y AcadSci 2001; 936 (01) 355-367
• 25 Suarez M, Haenni M, Canarelli S. et al. Structure-function characterization and optimization of a plant-derived antibacterial peptide. Antimicrob Agents Chemother 2005; 49 (09) 3847-3857
• 26 Moyo B, Masika PJ, Muchenje V. Antimicrobial activities of. Moringa oleifera . Lam leaf extracts. Afr J Biotechnol 2012; 11 (11) 2797-2802
• 27 Krishnaiah D, Devi T, Bono A, Sarbatly R. Studies on phytochemical constituents of six Malaysian medicinal plants. J Med Plants Res 2009; 3 (02) 067-072
• 28 Vinoth B, Manivasagaperumal R, Balamurugan S. Phytochemical analysis and antibacterial activity of. Moringa oleifera . Lam. Int J Res Biol Sci 2012; 2 (03) 98-102
• 29 Muhammad AA, Pauzi NA, Arulselvan P, Abas F, Fakurazi S. In vitro wound healing potential and identification of bioactive compounds from. Moringa oleifera . Lam. BioMed Res Int 2013. doi:10.1155/2013/974580