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CC BY 4.0 · Eur J Dent 2023; 17(01): 155-160
DOI: 10.1055/s-0042-1743154
Original Article

Malondialdehyde Level and Tissue Apoptosis Count as an Early-Detection Marker of Oral Potentially Malignant Disorders

Amalia Yosi Firdausa
1   Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Sally Salsabila Ahimsa
1   Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Rafdan Affan Ahmada 
1   Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Natasya Fauzia Sukmawati
1   Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Diah Savitri Ernawati
2   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya-Indonesia
,
Adiastuti Endah Parmadiati
2   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya-Indonesia
,
Bagus Soebadi
2   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya-Indonesia
,
Desiana Radithia 
2   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya-Indonesia
,
Saka Winias 
2   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya-Indonesia
,
Fatma Yasmin Mahdani
2   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya-Indonesia
,
Riyan Iman Marsetyo
2   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya-Indonesia
,
Rosnah Binti Zain 
3   Faculty of Dentistry, MAHSA University, Bandar Saujana Putra, Jenjarom Selangor, Malaysia
4   Oral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, Malaya, Malaysia
,
Nurina Febriyanti Ayuningtyas
2   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya-Indonesia
› Author Affiliations Funding This study was funded by the Faculty of Dental Medicine Universitas Airlangga in Schema Penelitian Unggulan Fakultas 2020.
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Abstract

Objectives The malondialdehyde (MDA) level and TA count represent the progression of oral potentially malignant disorders (OPMD) to malignancy and thus may be used as an indicator of oral epithelial dysplasia (OED). This study aimed to determine the MDA level and tissue apoptosis (TA) count in oropharyngeal tissue of Wistar rats exposed to sidestream cigarette smoke.

Materials and Methods Wistar rats were divided into three groups: T4 group (4-week cigarette smoke exposure), T8 group (8-week cigarette smoke exposure), and control group, which was not exposed to cigarette smoke. The oropharyngeal tissue of the rats from each group was examined histopathologically to count the number of apoptotic cells, and then the blood serum was made to measure the MDA level.

Statistical Analysis Bonferroni test was performed to see the differences in each group for MDA level. While the data from tissue apoptosis were analyzed using Mann-Whitney U test for the significance. All data were considered significant if p < 0.05.

Results The MDA level and TA count increased as the duration of cigarette smoke exposure increased. In the T8 group, the MDA level and TA count were significantly higher compared with the T4 and control groups with a p-value < 0.05.

Conclusions Exposure to sidestream cigarette smoke increased the TA count and MDA level in the oropharyngeal tissue of Wistar rats. The TA count and MDA level may be used as markers of OPMD.

Keywords

tobacco addiction - apoptosis index - malondialdehyde - oral epithelial dysplasia - oral potentially malignant disorder - cancer


Publication History

Article published online:
18 April 2022

© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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