The Response of the Tongue Epithelial on Cigarette Smoke Exposure as a Risk Factor for Oral Cancer Development

Abstract

Objective The aim of this study is to analyze the tongue epithelial response to cigarette smoke exposure on a number of macrophages, lymphocytes, plasma cells, and matrix metalloproteinase 9 (MMP-9) expression to determine the risk factor of oral cancer development.

Materials and Methods Thirty Rattus norvegicus will be exposed to two kinds of cigarette smoke by a smoking pump for 4 and 8 weeks. The tongues were collected to analyze the number of macrophages, lymphocytes, and plasma cells with hematoxylin-eosin. The MMP-9 expression was similarly analyzed with immunohistochemical staining and then compared with the control group.

Results The number of macrophages, lymphocytes, and MMP-9 expression was higher in the 8-week cigarette smoke exposure compared to the 4-week cigarette smoke exposure and the control group (p < 0.000). The number of plasma cell did not differ in the 8-week cigarette smoke exposure from that of the control group (p > 0.05). The number of plasma cells in the tongue tissue during the 4-week cigarette smoke exposure was not determined.

Conclusion Cigarette smoke exposure induces the risk of oral cancer development as a result of an increase in the number of macrophages, lymphocytes, and MMP-9 expression in the tongue epithelial.

Keywords

cigarette smoke - lymphocytes - macrophages - MMP-9 expression - tongue

Publikationsverlauf

Artikel online veröffentlicht:
07. Dezember 2020

• References

• 1 Idris S, Baqays A, Isaac A, Chau JKM, Calhoun KH, Seikaly H. The effect of second hand smoke in patients with squamous cell carcinoma of the head and neck. J Otolaryngol Head Neck Surg 2019; 48 (01) 33
• 2 Sierra H, Cordova M, Chen CJ, Rajadhyaksha M. Confocal imaging-guided laser ablation of basal cell carcinomas: an ex vivo study. J Invest Dermatol 2015; 135 (02) 612-615
• 3 Ghallab NA, Shaker OG. Serum and salivary levels of chemerin and MMP-9 in oral squamous cell carcinoma and oral premalignant lesions. Clin Oral Investig 2017; 21 (03) 937-947
• 4 Sood P, Narang R, Swathi V, Mittal L, Jha K, Gupta A. Dental patient’s knowledge and perceptions about the effects of smoking and role of dentists in smoking cessation activities. Eur J Dent 2014; 8 (02) 216-223
• 5 Patel JB, Shah FD, Joshi GM, Patel PS. Clinical significance of inflammatory mediators in the pathogenesis of oral cancer. J Cancer Res Ther 2016; 12 (02) 447-457
• 6 Valavanidis A, Vlachogianni T, Fiotakis K. Tobacco smoke: involvement of reactive oxygen species and stable free radicals in mechanisms of oxidative damage, carcinogenesis and synergistic effects with other respirable particles. Int J Environ Res Public Health 2009; 6 (02) 445-462
• 7 Sundar IK, Javed F, Romanos GE, Rahman I. E-cigarettes and flavorings induce inflammatory and pro-senescence responses in oral epithelial cells and periodontal fibroblasts. Oncotarget 2016; 7 (47) 77196-77204
• 8 Tampa M, Mitran MI, Mitran CI. et al. Mediators of inflammation: a potential source of biomarkers in oral squamous cell carcinoma. J Immunol Res 2018; 2018: 1061780
• 9 Ng WK, Wong SH, Ng SC. Changing epidemiological trends of inflammatory bowel disease in Asia. Intest Res 2016; 14 (02) 111-119
• 10 Goertzen C, Mahdi H, Laliberte C. et al. Oral inflammation promotes oral squamous cell carcinoma invasion. Oncotarget 2018; 9 (49) 29047-29063
• 11 Bhat TA, Kalathil SG, Bogner PN. et al. Secondhand smoke induces inflammation and impairs immunity to respiratory infections. J Immunol 2018; 200 (08) 2927-2940
• 12 Gracia I, Utoro T, Supriatno S, Astuti I, Heriyanto DS, Pramono D. Epidemiologic profile of oral squamous cell carcinoma in Yogyakarta, Indonesia. Padjadjaran J Dent 2017; 29 (01) 32-37
• 13 Venugopal A, Uma Maheswari TN. Expression of matrix metalloproteinase-9 in oral potentially malignant disorders: a systematic review. J Oral Maxillofac Pathol 2016; 20 (03) 474-479
• 14 Peschos D, Damala C, Stefanou D. et al. Expression of matrix metalloproteinase-9 (gelatinase B) in benign, premalignant and malignant laryngeal lesions. Histol Histopathol 2006; 21 (06) 603-608
• 15 Chandolia B, Basu SK, Kumar M. Can MMP-9 be a prognosticator marker for oral squamous cell carcinoma?. J Clin Diagn Res 2016; 10 (01) ZC09-ZC13
• 16 Lee J, Taneja V, Vassallo R. Cigarette smoking and inflammation: cellular and molecular mechanisms. J Dent Res 2012; 91 (02) 142-149
• 17 Zaquia Leão H, Galleano Zettler C, Cambruzzi E. et al. The effects of passive smoking on laryngeal and tracheal mucosa in male wistar rats during growth: an experimental study. J Voice 2017; 31 (01) 126.e19-126.e24
• 18 Ayuningtyas NF, Mahardika GO, Soebadi B. et al. Hyperplasia of Wistar rat tongue mucosa due to exposure to cigarette side-stream smoke. Dent J 2019; 52 (03) 133
• 19 Ayuningtyas NF, Endah A, Permatasari A. et al. The increase of dysplasia level in Wistar rats oropharyngeal mucosa exposed by sidestream cigarette smoke. EurAsian J Biosci. 2020; 14: 3641-3646
• 20 de Oliveira Semenzati G, de Souza Salgado B, Rocha NS, Michelin Matheus SM, de Carvalho LR, Garcia Martins RH. Histological and immunohistochemical study of the expression of p53 and ki-67 proteins in the mucosa of the tongue, pharynx and larynx of rats exposed to cigarette smoke. Inhal Toxicol 2012; 24 (11) 723-731
• 21 Qiu F, Liang C-L, Liu H. et al. Impacts of cigarette smoking on immune responsiveness: up and down or upside down?. Oncotarget 2017; 8 (01) 268-284
• 22 Loos BG, Roos MTL, Schellekens PTA. van der Velden U, Miedema F. Lymphocyte numbers and function in relation to periodontitis and smoking. J Periodontol 2004; 75 (04) 557-564
• 23 Hauff SJ, Raju SC, Orosco RK. et al. Matrix-metalloproteinases in head and neck carcinoma-cancer genome atlas analysis and fluorescence imaging in mice. Otolaryngol Head Neck Surg [Internet] 2014; 151 (04) 612-618
• 24 Irmawati A, Handayani ATW, Balqis NF, Surboyo MDC. The decreased of p53 mutant expression on squamous cell epithelial of oral in mus musculus by moderate intensity of exercise. Malaysian J Med Heal Sci. 2020; 16 (July) 1-5
• 25 Arundina I, Budhy TI, Surboyo MDC. Meloxicam inhibit the growth of oral squamous cell carcinoma induced by benzopyrenes. Malaysian J Med Heal Sci. 2020; 16 (04) 22-25
• 26 Fan H-X, Li H-X, Chen D, Gao Z-X, Zheng J-H. Changes in the expression of MMP2, MMP9, and ColIV in stromal cells in oral squamous tongue cell carcinoma: relationships and prognostic implications. J Exp Clin Cancer Res 2012; 31 (01) 90
• 27 Tsai C-H, Hsieh Y-S, Yang S-F, Chou M-Y, Chang Y-C. Matrix metalloproteinase 2 and matrix metalloproteinase 9 expression in human oral squamous cell carcinoma and the effect of protein kinase C inhibitors: preliminary observations. Oral Surg Oral Med Oral Pathol Oral Radiol Endod [Internet] 2003; 95 (06) 710-716
• 28 Murnane MJ, Cai J, Shuja S, McAneny D, Willett JB. Active matrix metalloproteinase-2 activity discriminates colonic mucosa, adenomas with and without high-grade dysplasia, and cancers. Hum Pathol 2011; 42 (05) 688-701