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CC BY 4.0 · Eur J Dent 2022; 16(04): 930-937
DOI: 10.1055/s-0042-1743145
Original Article

Polarized Microscopic Analysis of Picrosirius Red Stained Salivary Gland Pathologies: An Observational Study

Rachai Juengsomjit 
1   Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Ounruean Meesakul
1   Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Tawepong Arayapisit 
2   Department of Anatomy, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Noppadol Larbcharoensub
3   Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
,
Kajohnkiart Janebodin
2   Department of Anatomy, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
› Author Affiliations Funding This work was supported by the research funding from Faculty of Dentistry, Mahidol University.
› Further Information
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Abstract

Objective Salivary gland diseases and their pathologies may affect the glandular structure including collagen, a major stromal component, in response to tissue damage or diseases. This study aimed to examine the changes in collagens in different salivary gland diseases using polarized picrosirius red staining.

Materials and Methods The submandibular gland samples diagnosed as sialadenitis, chronic sclerosing sialadenitis, pleomorphic adenoma, adenoid cystic carcinoma, and mucoepidermoid carcinoma were stained with picrosirius red, Masson's trichrome, and anticollagen I staining. The quantity of collagens was examined and reported as a percentage of positive picrosirius red area. The maturity of collagens was studied with polarized light microscope and reported as a percentage of orange-red and yellow-green polarized collagens, representing the mature and immature collagens, respectively.

Statistical Analysis The % positive areas for picrosirius red representing the collagen amount among salivary gland diseases were analyzed by one-way analysis of variance with Tukey's test. The % orange-red and % yellow-green polarized areas representing the collagen maturity were analyzed by Kruskal–Wallis test and Mann–Whitney U test.

Results The malignant tumors, adenoid cystic carcinoma (29.92) and mucoepidermoid carcinoma (26.59), had higher significant percentage of positive picrosirius red area, compared with the benign tumor (14.56), chronic sclerosing sialadenitis (10.61), and sialadenitis (7.22) (p < 0.05). The percentages of orange-red polarized areas are 48.07, 39.6, 62.67, 83.75, and 76.05 in sialadenitis, chronic sclerosing sialadenitis, pleomorphic adenoma, adenoid cystic carcinoma, and mucoepidermoid carcinoma, respectively. This percentage tended to increase in the benign and malignant lesions with statistical difference, compared with the inflammatory lesions (p < 0.05). There was no statistical difference in the percentages of yellow-green polarized areas among various salivary gland diseases. In addition, the results of Masson's trichrome and anticollagen I staining are corresponding to that of picrosirius red among various salivary gland diseases.

Conclusions Polarized picrosirius red demonstrated the most amounts of collagen in the malignant lesion, and represented the different maturity of collagens in each lesion group. Studying the amounts and maturity of collagen with picrosirius red for extracellular matrix alteration in salivary gland diseases along with routine hematoxylin and eosin, Masson's trichrome, and immunohistochemistry may provide a better understanding in different salivary gland pathologies.

Keywords

salivary gland diseases - picrosirius red - polarized light - Masson's trichrome - collagen

Ethical Approval

The ethical approval was granted from the human ethical committee, Faculty of Dentistry and Pharmacy, Mahidol University, Bangkok, Thailand (COA.No.MU-DT/PY-IRB 2016/033.0906).




Publication History

Article published online:
17 May 2022

© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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