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CC BY 4.0 · Eur J Dent 2022; 16(04): 848-855
DOI: 10.1055/s-0041-1740226
Original Article

Topical Medicine Potency of Musa paradisiaca var. sapientum (L.) kuntze as Oral Gel for Wound Healing: An In Vitro, In Vivo Study

Hendrik Setia Budi 
1   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Silvia Anitasari
2   Department of Dental Material and Devices, Faculty of Medicine, Universitas Mulawarman, Samarinda, Indonesia
3   Department of Medical Microbiology, Faculty of Medicine, Universitas Mulawarman, Samarinda, Indonesia
,
Ninik Mas Ulfa
4   Surabaya Pharmacy Academy, Surabaya, Indonesia
,
Wisnu Setyari Juliastuti
1   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Mohammed Aljunaid 
5   Faculty of Dental Medicine, Alsaeed University, Taiz, Yemen
,
Doaa Elsayed Ramadan
6   Ministry of Health and Population, Cairo, Egypt
,
Koko Muzari
7   Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Yung-Kang Shen
8   School of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan
› Author Affiliations Funding This study was funded by Universitas Airlangga and the Indonesian Ministry of Research, Technology, and Higher Education.
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Abstract

Objective Topical application of ambonese banana (Musa paradisiaca var. sapientum (L.) kuntze) stem sap gel (GEGPA) on the socket wound area showed an increase in the expression of platelet-derived growth factor-BB, while decrease in the expression of matrix metalloproteinase-2 and 9. The aim of this study is to achieve standard formulation of GEGPA through stability, viscosity, distribution area, and drugs release for oral gel wound healing.

Materials and Methods This is an in vitro and in vivo study with the randomized posttest only control group design. The gel was formulated according to the composition of each group by adding hydroxypropyl methylcellulose (HPMC), Lexgard, propylene glycol, and cold water to obtain 100 g of gel. Observations were made through the following tests: stability, viscosity, distribution area, drug release, and histopathological analysis of tooth extraction wound healing.

Statistical analysis Data were analyzed using a one-way analysis of variance (α = 0.05) with GraphPad Prism-8 statistical software.

Results The study showed that the GEGPA formulation was stable against changes in consistency, color, smell, homogeneity, and pH value. There is a significant difference between groups with respect to viscosity (p = 0.0001), adhesion (p = 0.004), dispersion (p = 0.000), and fibroblast cell numbers on days 3 and 5 (p = 0.007 and p = 0.001). There is no interaction between the active ingredients and the gel base of all formulations. Formulation 3 had better properties in terms of viscosity, broad distribution, and drug release compared with other groups. Application of GEGPA to tooth extraction wounds showed a significant proliferation of fibroblast cells on days 3 and 5.

Conclusions The formulation of M. paradisiaca var. sapientum (L.) kuntze extract with HPMC and propylene glycol obtained a gel preparation, GEGPA, that was organoleptically stable and met the topical gel standard for wounds in the oral cavity.

Keywords

drug compounding - drug stability - health risk - oral gel - wound healing


Publication History

Article published online:
18 February 2022

© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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