Facial Plast Surg
DOI: 10.1055/s-0041-1730387
Original Research

The Effects of Vasonatrin Peptide on Fat Graft Viability: An Experimental Study

Fatih Irmak
1  Fatih Irmak Clinic, Istanbul, Turkey
,
2  Department of Plastic, Reconstructive and Aesthetic Surgery, Sisli Hamidiye Etfal Training and Research Hospital, Istanbul, Turkey
,
Selami Serhat Sirvan
2  Department of Plastic, Reconstructive and Aesthetic Surgery, Sisli Hamidiye Etfal Training and Research Hospital, Istanbul, Turkey
,
Semra Karsidag
2  Department of Plastic, Reconstructive and Aesthetic Surgery, Sisli Hamidiye Etfal Training and Research Hospital, Istanbul, Turkey
,
Aysim Ozagari
3  Department of Pathology, Sisli Hamidiye Etfal Training and Research Hospital, Istanbul, Turkey
› Author Affiliations
Funding This study was supported by Sağlık Bilimleri Üniversitesi Bilimsel Araştırma Projeleri, Istanbul Health Sciences University, Projects of Scientific Research, Istanbul (2018/047).

Abstract

Vasonatrin peptide (VNP) is a synthetic peptide that possesses vasodilatory, natriuretic, and anti-inflammatory properties. The authors aimed to analyze the effects of VNP on fat graft survival. Twenty Sprague–Dawley rats are randomly divided into two groups of 10. Fat grafts are harvested from the right inguinal region. After preparation, fat grafts are placed to the interscapular region. The first group of rats were administered VNP after their fat injection, while the second group received tail-vein injections of an equal volume of sterile saline following their fat injection. Experiment and control groups are evaluated according to their level of degeneration of adipocytes, fat necrosis, vacuolization, cyst formation in adipocytes, fibrosis of the fat tissue, capillary density, and CD31 immunohistochemical staining. Degeneration, vacuolization, and cyst formation in adipocytes were lower in the experiment group. Increased capillary density in the experiment group was demonstrated by CD31 antibody staining and by counting capillary density under a microscope. The average percentage of change in weight of the fat grafts in the experiment group was lower than that in the control group. The results indicate that VNP has some beneficial effects on fat graft survival by multiple independent mechanisms that influence both local and systemic homeostasis.



Publication History

Publication Date:
07 June 2021 (online)

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