Am J Perinatol
DOI: 10.1055/s-0041-1730349
Original Article

Evaluation of the Neuroprotective Effect of Pycnogenol in a Hypoxic-Ischemic Brain Injury Model in Newborn Rats

1   Neonatal Intensive Care Unit, Division of Pediatric, Medical Park Bahcelievler Hospital, Altınbas University Medical Faculty, Istanbul, Turkey
,
2   Department of Laboratory Animal Science, Faculty of Health Sciences, Dokuz Eylül University, Izmir, Turkey
,
3   Department of Pathology, Medical Faculty, Izmir Democracy University, Izmir, Turkey
,
4   Neonatal Intensive Care Unit, Dr. Behçet Uz Children's Education and Research Hospital, Izmir, Turkey
,
2   Department of Laboratory Animal Science, Faculty of Health Sciences, Dokuz Eylül University, Izmir, Turkey
,
4   Neonatal Intensive Care Unit, Dr. Behçet Uz Children's Education and Research Hospital, Izmir, Turkey
› Author Affiliations

Abstract

Objective This study aimed to evaluate the efficacy of Pycnogenol (PYC) and its antioxidant and antiapoptotic effect in an experimental hypoxic-ischemic (HI) rat model.

Study Design A total of 24 Wistar albino rats who were on the seventh postnatal day were divided into three groups with developed HI brain injury model under the sevoflurane anesthesia: 40 mg/kg PYC was given to Group A, saline was given to Group B, and the sham group was Group C. Neuronal apoptosis was investigated by terminal deoxynucleotidyl transferase dUTP nick end labeling and immunohistochemically stained manually with primer antibodies of tumor necrosis factor-α and interleukin-1β.

Results The neuronal cell injury was statistically lower in the PYC treatment group.

Conclusion This is the first study that investigates the role of PYC in the HI brain injury model. PYC reduces apoptosis and neuronal injury in the cerebral tissue of the rats. PYC may be a protective agent against hypoxic-ischemic encephalopathy.

Key Points

  • This is the first study that investigates the role of PYC in the HI brain injury model.

  • PYC may be a protective agent against hypoxic-ischemic encephalopathy.

  • Sevoflurane should not be preferred in rat studies where neuronal apoptosis will be investigated.

Authors' Contributions

R.C. designed the study and wrote the manuscript. A.C., S.A.O., and O.Y. designed the experimental model. G.D. evaluated the histopathological analysis. S.C. revised the manuscript.




Publication History

Received: 07 February 2021

Accepted: 05 April 2021

Article published online:
27 May 2021

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