Immunomodulation in Cholesteatoma

Immunomodulation in Cholesteatoma Introduction The etiopathogenesis of chronic otitis media epitympanalis and its proliferative destructive course with possible complications such as destruction of bony structures with hearing loss, vestibular dysfunction, facial nerve paralysis and intracranial complications are still unexplained. Surgery is still the way to go. New studies are increasingly looking at the innate immune system.

Methods Our studies were carried out on HMECC cells, in a mouse model in TNF/--mice; Cholestatom and healthy ear canal skin was removed during ear surgery. Moreover monocyte subsets of patients were evaluated versus healthy donors. The expression analyses were carried out at the gene and protein level, using Affymetrix Microarray and qPCR with TNF as the major target for therapy evaluation. By means of TUNEL staining, immunohistochemistry on cryocuts and in Situ Apoptosis Detection Kit, the level of apoptose by TNF was evaluated.

Results The uncontrolled undirected cholestatoma growth shows an immunomodulatory profile with high regulation of various gene networks, especially TNF downstream and upstream signaling pathways and their molecules. TNF is modelled both inflammatory and apoptotically and therefore is suitable as a possible therapeutic approach in various models.

Conclusions Cholestatom is immunomodulatory regulated.

Poster-PDF A-1563.pdf

Einzelförderung der Medizinischen Fakultät der Universität zu Lübeck, E37- (AL).

Publikationsverlauf

Artikel online veröffentlicht:
13. Mai 2021