CC BY 4.0 · Journal of Child Science 2020; 10(01): e187-e195
DOI: 10.1055/s-0040-1718896
Original Article

Measurement of Serum Chemerin, Oxidized LDL, and Vitamin D Levels in Prader–Willi Syndrome: A Cross-Sectional Study in Pediatric Egyptian Patients

Manal M. Thomas
1  Clinical Genetics Department, Center of Scientific Excellence, National Research Centre, Cairo, Egypt
,
Moushira E. Zaki
2  Department of Biological Anthropology, National Research Centre, Cairo, Egypt
,
Eman Youness
2  Department of Biological Anthropology, National Research Centre, Cairo, Egypt
,
Khaled Hamed
1  Clinical Genetics Department, Center of Scientific Excellence, National Research Centre, Cairo, Egypt
,
Azzah A. Khedr
3  Human Genetics and Genome Research Division, Human Cytogenetics Department, National Research Centre, Cairo, Egypt
,
Phoebe M. Abd El-Massieh
4  Human Genetics and Genome Research Division, Oro-dental Genetics Department, National Research Centre, Cairo, Egypt
,
Sara M. Abdo
5  Biochemistry Division, Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt
,
Hala T. El-Bassyouni
1  Clinical Genetics Department, Center of Scientific Excellence, National Research Centre, Cairo, Egypt
› Author Affiliations

Abstract

Prader–Willi syndrome (PWS) is the commonest genetic cause of obesity. Oxidative stress and chronic low-grade inflammation play a crucial role in the pathogenesis of obesity. Alterations of vitamin D (25-OHD) levels are commonly encountered with obesity. The aim of this study was to analyze serum chemerin, oxidized low-density lipoprotein (ox-LDL), and 25-OHD values in pediatric PWS patients in comparison with obese healthy children and nonobese control groups, highlighting possible correlations with body mass index (BMI) and obesity. Twenty-six PWS Egyptian patients and 26 obese healthy individuals referred to the outpatient clinic of the Clinical Genetics Department, National Research Centre, Cairo, Egypt, and 20 control patients with matching age and sex were enrolled in the study. Patients were clinically diagnosed and confirmed by routine cytogenetic and fluorescence in-situ hybridization analysis. Anthropometric measurements were performed, and BMI was calculated by weight/height2 (kg/m2), and BMI z score was also determined. Serum chemerin, ox-LDL, and vitamin D were determined by enzyme-linked immunosorbent assay. Chemerin levels, which reflected chronic inflammation, were significantly elevated as compared with obese and nonobese controls (p ≤ 0.0001). Concerning oxidative damage, children with PWS showed higher Ox-LDL levels compared with obese and nonobese controls (p < 0.0001). Vitamin D levels were significantly lower in PWS patients compared with obese and nonobese controls (p ≤ 0.0001). Our data showed that obesity in PWS is associated with oxidative stress and chronic low-grade inflammation. Ox-LDL is a good indicator of oxidative stress, and chemerin could be used as a biomarker for the chronic inflammatory state. Furthermore, vitamin D supplementation is recommended in PWS patients



Publication History

Received: 10 July 2020

Accepted: 16 September 2020

Publication Date:
02 November 2020 (online)

© 2020. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/).

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