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CC BY-NC-ND 4.0 · AJP Rep 2020; 10(03): e335-e341
DOI: 10.1055/s-0040-1715166
Case Report

Mesenchymal Stem Cells Attenuate Lipopolysaccharide-Induced Inflammatory Response in Human Uterine Smooth Muscle Cells

Arunmani Mani
1   Division of Maternal Fetal Medicine, Department of Obstetrics, Gynecology and Reproductive Sciences, UT Health-McGovern Medical School, Houston, Texas
,
John W. Hotra
1   Division of Maternal Fetal Medicine, Department of Obstetrics, Gynecology and Reproductive Sciences, UT Health-McGovern Medical School, Houston, Texas
,
Sean C. Blackwell
1   Division of Maternal Fetal Medicine, Department of Obstetrics, Gynecology and Reproductive Sciences, UT Health-McGovern Medical School, Houston, Texas
,
Laura Goetzl
1   Division of Maternal Fetal Medicine, Department of Obstetrics, Gynecology and Reproductive Sciences, UT Health-McGovern Medical School, Houston, Texas
,
Jerrie S. Refuerzo
1   Division of Maternal Fetal Medicine, Department of Obstetrics, Gynecology and Reproductive Sciences, UT Health-McGovern Medical School, Houston, Texas
› Institutsangaben Funding The study was supported from the Department of Obstetrics, Gynecology, and Reproductive Sciences, UTHealth-McGovern Medical School, Houston TX and The Larry C. Gilstrap, MD, Center for Perinatal and Women's Health Research, University of Texas Health Science Center at Houston.
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Abstract

Objective The aim of this study was to determine if mesenchymal stem cells (MSCs) would suppress the inflammatory response in human uterine cells in an in vitro lipopolysaccharide (LPS)-based preterm birth (PTB) model.

Study Design Cocultures of human uterine smooth muscle cells (HUtSMCs) and MSCs were exposed to 5 μg/mL LPS for 4 hours and further challenged with 1 μg/mL LPS for a subsequent 24 hours. Key elements of the parturition cascade regulated by toll-like receptors (TLRs) through activation of mitogen-activated protein kinases (MAPKs) were quantified in culture supernatant as biomarkers of MSC modulation.

Results Coculture with MSCs significantly attenuated TLR-4, p-JNK, and p- extracellular signal-regulated kinase 1/2 (ERK1/2) protein levels compared with HUtSMCs monoculture (p = 0.05). In addition, coculture was associated with significant inhibition of proinflammatory cytokines interleukin (IL)-6 and IL-8 (p = 0.0001) and increased production of anti-inflammatory cytokines IL-10 and transforming growth factor (TGF)-β1 (p = 0.0001).

Conclusion MSCs appear to play a role in significantly attenuating LPS-mediated inflammation via alteration of down-stream MAPKs. MSCs may represent a novel, cell-based therapy in women with increased risk of inflammatory-mediated preterm birth.

Keywords

mesenchymal stem cells - lipopolysaccharide - inflammatory response - cytokines - preterm birth

Note

This study was presented as Posters at the 38th Annual Meeting of the Society for Maternal-Fetal Medicine; January 29 to February 3, 2018; Dallas, TX, and at the 65th Annual Scientific Meeting of the Society for Reproductive Investigation; March 6 to 10, 2018 in San Diego, CA.




Publikationsverlauf

Eingereicht: 27. März 2020

Angenommen: 24. April 2020

Artikel online veröffentlicht:
23. September 2020

© 2020. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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