J reconstr Microsurg
DOI: 10.1055/s-0039-3401036
Original Article
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Faster Detection of Ischemia in Free Muscle Transfer When Using Microdialysis

Andreas Rauff-Mortensen
1  Institute of Clinical Medicine, Aarhus University, Aarhus, Denmark
2  Research Center for Emergency Medicine and Department of Anaesthesiology and Intensive Care Medicine, Aarhus University Hospital and Aarhus University, Aarhus, Denmark
,
Mette Marie Berggren-Olsen
3  Department of Vascular Surgery, Kolding Hospital, Kolding, Denmark
4  Institute of Regional Health Research, University of Southern Denmark, Odense, Denmark
,
Hans Kirkegaard
2  Research Center for Emergency Medicine and Department of Anaesthesiology and Intensive Care Medicine, Aarhus University Hospital and Aarhus University, Aarhus, Denmark
5  Research Center for Emergency Medicine, Aarhus University Hospital, Aarhus, Denmark
,
Kim Houlind
3  Department of Vascular Surgery, Kolding Hospital, Kolding, Denmark
4  Institute of Regional Health Research, University of Southern Denmark, Odense, Denmark
,
Hanne Birke-Sørensen
1  Institute of Clinical Medicine, Aarhus University, Aarhus, Denmark
3  Department of Vascular Surgery, Kolding Hospital, Kolding, Denmark
› Author Affiliations
Further Information

Publication History

12 June 2019

28 October 2019

Publication Date:
13 December 2019 (online)

Abstract

Background Microdialysis is a clinical method used to detect ischemia after microvascular surgery. Microdialysis is easy to use and reliable, but its value in most clinical settings is hampered by a 1- to 2-h delay in the delivery of patient data. This study evaluated the effectiveness of an increase in the microdialysis perfusion rate from 0.3 to 1.0 µL/min on the diagnostic delay in the detection of ischemia.

Methods In eight pigs, two symmetric pure muscle transfers were dissected based on one vascular pedicle each. In each muscle, two microdialysis catheters were placed. The two microdialysis catheters were randomized to a perfusion rate of 0.3 or 1.0 µL/min, and the two muscle transfers were randomized to arterial or venous ischemia, respectively. After baseline monitoring, arterial and venous ischemia was introduced by the application of vessel clamps. Microdialysis sampling was performed throughout the experiment. The ischemic cutoff values were based on clinical experience set as follows: CGlucose < 0.2 mmol/L, CLactate > 7 mmol/L, and the lactate/pyruvate ratio > 50.

Results The delay for the detection of 50% of arterial ischemia was reduced from 60 to 25 minutes, and for the detection of all cases of arterial ischemia, the delay was reduced from 75 to 40 minutes when the perfusion rate was increased from 0.3 to 1.0 µL/min. After the same increase in perfusion, the detection of 50% of venous ischemia was reduced from 75 to 40 minutes, and for all cases of venous ischemia, a reduction from 135 to 95 minutes was found.

Conclusion When using microdialysis for the detection of ischemia in pure muscle transfers, an increase in the perfusion rate from 0.3 to 1.0 µL/min can reduce the detection delay of ischemia.