CC BY-NC-ND 4.0 · Journal of Morphological Sciences 2019; 36(04): 219-222
DOI: 10.1055/s-0039-3400482
Original Article
Thieme Revinter Publicações Ltda Rio de Janeiro, Brazil

Cryodehydration Technique Applied to Anatomical Segments

Althen Teixeira Filho
1  Department of Morphology, Institute of Biology, Universidade Federal de Pelotas, Capão do Leão, RS, Brazil
,
Bárbara Tavares Schäfer
2  Department of Surgery, School of Veterinary Medicine and Animal Science, Universidade de São Paulo, São Paulo, SP, Brazil
,
Patrícia Silva Vives
3  Hospital of Veterinary Clinics, College of Veterinary, Universidade Federal de Pelotas, Capão do Leão, RS, Brazil
› Author Affiliations
Further Information

Publication History

10 November 2017

17 October 2019

Publication Date:
04 December 2019 (online)

  

Abstract

Introduction The present study describes a variation of the cryodehydration technique, applied to prepare biological tissues by repetitions of section freezing and thawing (SFT). First developed for muscles and then viscera, the aim of this process as presented here is to obtain anatomical segments to be used in anatomy classes, general exhibitions and museums.

Materials and Methods The animal, previously fixed in 10% formalin, must be frozen and then sectioned in longitudinal, transverse and horizontal slices of the body-axis. It has two steps: the “burning phase,” in which ice crystal formation and water dilatation cause micro ruptures in the tissue; and the “dehydration phase,” developed to dry out the slices segments slowly and, at the same time, to impregnate tissues with wood glue.

Results The obtained slices present several advantages, such as being dry segments, of light weight, and being rapidly used, easily stored and promptly studied. Also, it is possible to study the different organs of one segment and, by apposition, remount an entire animal, enabling a dynamic study.

Conclusion This very simple and inexpensive technique produces anatomical preparations with high durability and resistance, which are very helpful in practical and theoretical classes.