Planta Med 2019; 85(18): 1489-1490
DOI: 10.1055/s-0039-3399898
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

Isolation of three flavonoids and a phenolic compound from Macaranga hypoleucaei

HM Nazeri
1   Faculty of Applied Science, UiTM Shah Alam,, 40450 Shah Alam, Selangor D.E. Malaysia
2   Atta-ur-Rahman Institute for Natural Product Discovery, Universiti Teknologi MARA, Puncak Alam Campus,, 42300 Bandar Puncak Alam, Selangor D.E. Malaysia
,
N Ahmat
1   Faculty of Applied Science, UiTM Shah Alam,, 40450 Shah Alam, Selangor D.E. Malaysia
2   Atta-ur-Rahman Institute for Natural Product Discovery, Universiti Teknologi MARA, Puncak Alam Campus,, 42300 Bandar Puncak Alam, Selangor D.E. Malaysia
,
AS Kamarozaman
1   Faculty of Applied Science, UiTM Shah Alam,, 40450 Shah Alam, Selangor D.E. Malaysia
2   Atta-ur-Rahman Institute for Natural Product Discovery, Universiti Teknologi MARA, Puncak Alam Campus,, 42300 Bandar Puncak Alam, Selangor D.E. Malaysia
3   Centre of Foundation Studies, Universiti Teknologi MARA, Selangor Branch, Dengkil Campus,, 43800 Dengkil, Selangor, Malaysia
,
NFN Azmin
1   Faculty of Applied Science, UiTM Shah Alam,, 40450 Shah Alam, Selangor D.E. Malaysia
,
NKNA Zawawi
1   Faculty of Applied Science, UiTM Shah Alam,, 40450 Shah Alam, Selangor D.E. Malaysia
,
SA Syed Mohamad
1   Faculty of Applied Science, UiTM Shah Alam,, 40450 Shah Alam, Selangor D.E. Malaysia
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

 

Macaranga (mahang) belongs to the Euphorbiaceae family with over 300 species and is native to tropical Africa, Madagascar, South-East Asia, Australia and the Pacific region [1]. The leaves of many Macaranga species are used to treat swelling, fresh cuts, sores, bruises and boils [2]. This genus is known as a source of flavonoids and stilbenoids which possess various biological activities [3]. Powdered leaves of M. hypoleuca (Reichb.F. & Zoll.) Müll. Arg. (2.5 kg) were macerated in methanol for 24 h at room temperature three times. The crude extract (700 g) was subjected to liquid-liquid partition using n-hexane and ethyl acetate. The ethyl acetate fraction (370 g) was fractionated using vacuum liquid chromatography (VLC) to give eight major fractions (HL1-HL8). Fraction HL5 (2.34 g) was subjected to fractionation to yield seven fractions (HL51-HL57). Fraction HL54 was further purified using column chromatography (CC) and preparative thin layer chromatography (p-TLC) to get one pure compound. Fraction HL6 (10.6 g) was fractionated using VLC to give eight fractions (HL61-HL68). Fraction HL65 and HL66 were subjected for fractionation and purification using column CC, high performance liquid chromatography (HPLC) and p-TLC to afford three pure compounds. The pure compounds were analyzed based on their NMR, UV-Vis and IR data as well as comparison with literature data. A flavanone tomentosanol D (1) (5.8 mg), two flavonols namely quercetin (2) (26.4 mg) and kaempferol (3) (6 mg), as well as 3,4-dihydroxybenzoic acid (4) (2 mg) were purified successfully from the leaves of M. hypoleuca.

Zoom Image
Fig. 1
 

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