Planta Med 2019; 85(18): 1438-1439
DOI: 10.1055/s-0039-3399766
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

Optimization of extraction condition for flavonoids in Genkwa Flos using response surface methodology

G Park
1   Kyung Hee University,, Department of Biomedical Science and Technology, Graduate School, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Korea
,
E Choi
2   Kyung Hee University,, Department of Life and Nanopharmaceutical Sciences, Graduate School, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Korea
,
M Kim
2   Kyung Hee University,, Department of Life and Nanopharmaceutical Sciences, Graduate School, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Korea
,
SC Park
2   Kyung Hee University,, Department of Life and Nanopharmaceutical Sciences, Graduate School, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Korea
,
YP Jang
2   Kyung Hee University,, Department of Life and Nanopharmaceutical Sciences, Graduate School, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Korea
3   Kyung Hee University,, Department of Oriental Pharmaceutical Science, College of Pharmacy, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Korea
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

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Genkwa Flos is the flower buds of Daphne genkwa Sieb. et Zucc. (Thymelaeaceae)a traditional medicine in Korea and China [1], [2]. enkwanin is characterized as the reference phytochemical for the quality control of this herbal medicine in Chinses and Taiwan Pharmacopoeia. ive flavonoids including genkwanin in the Genkwa Flos using ultra-performance liquid chromatography coupled with a photodiode array detector. o optimize the condition of extraction, which mapped out a mixture matrix design (MMD) for extract solvent and three-level full factorial design with regression analysis [3]. Water, methanol, and acetone were as the extraction solvents for MMD and the extraction method. Following multiple regression analyses of, 3D response surface and the contour plots to determine the optimal conditions. extractsolvent44% water, 56% acetone, and 0% methanol12 hours150 rpm70℃. This work proposed a method for the optimal extraction of flavonoids in Genkwa Flos and the determination of five flavonoids apigenin and genkwanin by UPLC-DAD.

 

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