Comparative Effect of Calcium Phosphate Biocomposite Materials on the Healing of Experimental Defect of Compact Bone Tissue

Abstract

Introduction For the treatment of bone defects, a considerably large number of biocomposite calcium-phosphate materials has been developed and used. However, in the scientific literature, there is no information about the comparative effect of biocomposite materials based on β-tricalcium phosphate, hydroxyapatite, and collagen on the dynamics of healing of the defect of compact bone tissue.

Materials and Methods The experiment was performed on 48 white Wistar rats. In the middle third of the femoral shaft, a perforated defect 2.5 mm in diameter was reproduced in the bone marrow canal, which was filled with the calcium phosphate material Collapan (Intermedapatit, Moscow, Russia) (hydroxyapatite/collagen/antibiotics) in the animals of the first group, and with Guidor easy-graft Crystal (Sunstar S.A., Etoy, Switzerland) (hydroxyapatite/β-tricalcium phosphate) in the animals of the second group. Fragments of the injured bones were examined on the 15^th and 30^th days by light microscopy with morphometry and scanning electron microscopy.

Results It was found that, in the area of implantation of Collapan and of Guidor easy-graft Crystal, signs of inflammation were not detected, and osteogenic cells exhibited high topism to biocomposite materials. The biomaterials during the entire period of the experiment are subjected to resorption and replacement by the bone tissue of the regenerate. On the 15^th and 30^th days of the experiment, the predominance in the rate of biomaterial resorption (of 35.04% and 53.47%, respectively) and the formation of regenerate bone tissue (of 58.67% and 50.47%, respectively) was in the area of implantation of Collapan.

Conclusion The biocomposite materials tested exhibit a high biocompatibility, osteoconductive properties, and good integration with the tissues of the regenerate. However, the biocomposite material Collapan undergoes resorption and replacement by the bone tissue of the regenerate much faster, and Guidor easy-graft Crystal provides stability of the defect volume of compact bone tissue due to full resorption and good integration with the tissues of the regenerate.

Keywords

rats - reparative osteogenesis - β-tricalcium phosphate - hydroxyapatite - collagen

• References

• 1 Pankratov AS, Lekishvili MV, Kopetsky IS. Bone grafting in dentistry and maxillofacial surgery. Osteoplastic materials: A Guide for Physicians. Moscow: BINOM; 2011
• 2 Cohen C, Bernard R. Endodontics. Saint Petersburg: Interline; 2000
• 3 Daculsi G, Laboux O, Malard O, Weiss P. Current state of the art of biphasic calcium phosphate bioceramics. J Mater Sci Mater Med 2003; 14 (03) 195-200
• 4 Köhli M. Evaluation of moldable in situ hardening bone graft substitute in an animal model. Implants extra international magazine of oral implantology. Bone regeneration. Special Edition Degradable Solutions AG 2012; 01: 5-34
• 5 Jensen SS, Bornstein MM, Dard M, Bosshardt DD, Buser D. Comparative study of biphasic calcium phosphates with different HA/TCP ratios in mandibular bone defects. A long-term histomorphometric study in minipigs. J Biomed Mater Res B Appl Biomater 2009; 90 (01) 171-181
• 6 LeGeros RZ, Lin S, Rohanizadeh R, Mijares D, LeGeros JP. Biphasic calcium phosphate bioceramics: preparation, properties and applications. J Mater Sci Mater Med 2003; 14 (03) 201-209
• 7 Malard O, Guicheux J, Bouler JM. , et al. Calcium phosphate scaffold and bone marrow for bone reconstruction in irradiated area: a dog study. Bone 2005; 36 (02) 323-330
• 8 Lan Levengood SK, Polak SJ, Wheeler MB. , et al. Multiscale osteointegration as a new paradigm for the design of calcium phosphate scaffolds for bone regeneration. Biomaterials 2010; 31 (13) 3552-3563
• 9 Zafiropoulos GG, Hoffmann O, Kasaj A, Willershausen B, Weiss O, van Dyke TE. Treatment of intrabony defects using guided tissue regeneration and autogenous spongiosa alone or combined with hydroxyapatite/beta-tricalcium phosphate bone substitute or bovine-derived xeno-graft. J Periodontol 2007; 78 (11) 2216-2225
• 10 Dorozhkin SV. Calcium orthophosphate-containing biocomposites and hybrid biomaterials for biomedical applications. J Funct Biomater 2015; 6 (03) 708-832
• 11 Lindgren C, Hallman M, Sennerby L, Sammons R. Back-scattered electron imaging and elemental analysis of retrieved bone tissue following sinus augmentation with deproteinized bovine bone or biphasic calcium phosphate. Clin Oral Implants Res 2010; 21 (09) 924-930
• 12 Schmidlin PR, Nicholls F, Kruse A, Zwahlen RA, Weber FE. Evaluation of moldable, in situ hardening calcium phosphate bone graft substitutes. Clin Oral Implants Res 2013; 24 (02) 149-157
• 13 Tsuchiya A, Sotome S, Asou Y. , et al. Effects of pore size and implant volume of porous hydroxyapatite/collagen (HAp/Col) on bone formation in a rabbit bone defect model. J Med Dent Sci 2008; 55 (01) 91-99
• 14 Jenkins MJ. Polymers in biology and medicine. Moscow. Sci World 2011
• 15 Germanov VG, Kovalersky GM, Cherkashena ZA, Semenov VA. Osteoplastic surgery: From bone transplant to the modern biocomposite materials. Medical Assistance Available 2006; 04: 16-19
• 16 Iordanishvili AK, Gololobov VG, Baschenko YV, Sakharov NV. Collapan – a modern optimizer of reparative osteogenesis. Ambulatory surgery. Hospital Replacing Technologies 2002; 2: 6-8
• 17 Ruffieux K, Kohli M. Build-up of bone tissue. Materials research and clinical application instructions easy-graft® materials and easy-graft®CRYSTAL. Kiev: Company “Stam”; 2011
• 18 Berchenko GN, Kesyan GA, Urazgildeev RZ, Arsenyev IG, Mikelaishvili DS. Comparative experimental morphological study of the effect of some calcium phosphate materials used in trauma and orthopedic practice to activate reparative osteogenesis. Bulletin of the East Siberian Scientific Center. Siberian Branch of Russian Academy of Medical Sciences 2006; 4: 327-333
• 19 Protsenko AI, Nikuradze VK. Collap An in surgery of injuries and diseases of the cervical spine. Moscow: Typography HT-print; 2013
• 20 Yuehuei HA, Kylie LM. Handbook of Histology Methods for Bone and Cartilage. Totowa, New Jersey: Humana Press; 2003
• 21 Grigorian AS, Toporkova AK. Problems of integration of implants in bone tissue (theoretical aspects). Moscow: Technosphere; 2007
• 22 Barer GM, Vavilov TP, Tumanov AS. The use of the biocomposite drug “CollapAn” containing various antibacterial inclusions on the surgical stage of complex treatment of periodontitis. Kafedra 2004; 10: 45-49
• 23 Snetkov AI, Batrak SY, Frantov AR, Avakian AM. Applications of implant “CollapAn” in the clinic of pediatric bone pathology. Biomaterials 2006; 5: 2-4
• 24 Glaser R. Rehabilitation mit 3D Planung und Kieferkammerhalt. Zahnarzt 2009; 04: 29-31
• 25 Semenyak SA, Rublenko MV, Dudka VB. Clinical and morphologic characteristics using of osteotropic materials to replace bone defects in animals. Vet Med 2015; 100: 157-161