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CC BY-NC-ND 4.0 · Journal of Morphological Sciences 2018; 35(03): 173-176
DOI: 10.1055/s-0038-1673611
Short Communication
Thieme Revinter Publicações Ltda Rio de Janeiro, Brazil

Anatomopathological Assessment of the Diaphragm in Formalin-Fixed, Paraffin-Embedded Sections

Avaliação anatomopatológica do diafragma em cortes fixados em formalina e incorporados em parafina
Ricardo Aparecido Baptista Nucci
1   Department of Pathology, Faculty of Medicine, Universidade de São Paulo, São Paulo, SP, Brazil
2   Postgraduate Pathology program, Faculty of Medicine, Universidade de São Paulo, São Paulo, SP, Brazil
,
Wilson Jacob-Filho
2   Postgraduate Pathology program, Faculty of Medicine, Universidade de São Paulo, São Paulo, SP, Brazil
3   Department of Geriatrics, Faculty of Medicine, Universidade de São Paulo, São Paulo, SP, Brazil
,
Alexandre Leopold Busse
2   Postgraduate Pathology program, Faculty of Medicine, Universidade de São Paulo, São Paulo, SP, Brazil
3   Department of Geriatrics, Faculty of Medicine, Universidade de São Paulo, São Paulo, SP, Brazil
,
Laura Beatriz Mesiano Maifrino
4   Laboratory of Morphoquantitative Studies and Immunohistochemistry, Department of Physical Education, Universidade São Judas Tadeu, São Paulo, SP, Brazil
5   Postgraduate Physical Education program, Universidade São Judas Tadeu, São Paulo, SP, Brazil
,
Romeu Rodrigues de Souza
6   Department of Anatomy, Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, SP, Brazil
7   Postgraduate Aging Sciences program, Universidade São Judas Tadeu, São Paulo, SP, Brazil
› Author Affiliations
Further Information

Publication History

12 February 2018

09 August 2018

Publication Date:
11 October 2018 (online)

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Abstract

Introduction The analysis of frozen muscle biopsies has become a routine method in the evaluation of muscle structure in health and disease. However, the technique for frozen muscle specimens is not widely available in countries with limited medical facilities. The present study aimed to elucidate a reproducible formalin-fixed and paraffin-embedded (FFPE) method for this type of analysis in postmortem muscles.

Methods Diaphragm muscle was obtained within 1 hour of sudden death. Diaphragm strips were washed in saline solution, fixed in 10% formalin, frozen at 4°C in a refrigerator, and stored for 24 hours. Then, the tissue samples were processed into paraffin-embedded blocks. Transversal sections were cut from each paraffin block and stained with hematoxylin and eosin, Picrosirius red, Verhoeff-Van Gieson, and Congo red for the qualitative analysis.

Results Our analysis indicated a well-preserved muscle.

Conclusion In summary, we demonstrate a simple technique for a reproducible FFPE method in postmortem muscle tissues.

Keywords

anatomy - muscle - necropsy - pathology